Print Email Facebook Twitter A role for NF-KB-dependent gene transactivation in sunburn Title A role for NF-KB-dependent gene transactivation in sunburn Author Abeyama, K. Eng, W. Jester, J.V. Vink, A.A. Edelbaum, D. Cockerell, C.J. Bergstresser, P.R. Takashima, A. Publication year 2000 Abstract Exposure of skin to ultraviolet (UV) radiation is known to induce NF-κB activation, but the functional role for this pathway in UV-induced cutaneous inflammation remains uncertain. In this study, we examined whether experimentally induced sunburn reactions in mice could be prevented by blocking UV-induced, NF-κB-dependent gene transactivation with oligodeoxynucleotides (ODNs) containing the NF-κB cis element (NF-κB decoy ODNs). UV-induced secretion of IL-1, IL-6, TNF-α, and VEGF by skin-derived cell lines was inhibited by the decoy ODNs, but not by the scrambled control ODNs. Systemic or local injection of NF-κB decoy ODNs also inhibited cutaneous swelling responses to UV irradiation. Moreover, local UV-induced inflammatory changes (swelling, leukocyte infiltration, epidermal hyperplasia, and accumulation of proinflammatory cytokines) were all inhibited specifically by topically applied decoy ODNs. Importantly, these ODNs had no effect on alternative types of cutaneous inflammation caused by irritant or allergic chemicals. These results indicate that sunburn reactions culminate from inflammatory events that are triggered by UV-activated transcription of NF-κB target genes, rather than from nonspecific changes associated with tissue damage. Subject immunoglobulin enhancer binding proteininterleukin 1interleukin 6oligodeoxynucleotidetumor necrosis factor alphavasculotropinoligodeoxyribonucleotideanimal experimentanimal modelarticlecytokine productiondisease associationmousenonhumanpathogenesispriority journalskin inflammationsunburntransactivationultraviolet irradiationA J mouseanimalBagg albino mousecell linechemistrycytologyedemafemalegene expression regulationgeneticshyperplasiakeratinocyteLangerhans cellmetabolismnucleotide sequencepathologypathophysiologyradiation exposureskinultraviolet radiationAnimalsBase SequenceCell LineEdemaFemaleGene Expression RegulationHyperplasiaKeratinocytesLangerhans CellsMiceMice, Inbred AMice, Inbred BALB CNF-kappa BOligodeoxyribonucleotidesSkinSunburnTrans-Activation (Genetics)Ultraviolet Rays To reference this document use: http://resolver.tudelft.nl/uuid:e0c57f04-abf5-4c38-b983-1f69bc416ecd TNO identifier 56740 Source The Journal of Clinical Investigation, 105 (12), 1751-1759 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.