Print Email Facebook Twitter Steviol Glycoside Rebaudioside A Induces Glucagon-like Peptide-1 and Peptide YY Release in a Porcine ex Vivo Intestinal Model Title Steviol Glycoside Rebaudioside A Induces Glucagon-like Peptide-1 and Peptide YY Release in a Porcine ex Vivo Intestinal Model Author Ripken, D. van der Wielen, N. Wortelboer, H.M. Meijerink, J. Witkamp, R.F. Hendriks, H.F.J. Publication year 2014 Abstract Glucagon-like peptide-1 (GLP-1) and peptide YY (PYY) are hormones important for satiation and are involved in the process called “ileal brake”. The aim of this study was to investigate the GLP-1- and PYY-stimulating efficacy of rebaudioside A, casein, and sucrose. This was studied using tissue segments collected from various regions of the pig small intestine. GLP-1 release was strongest from the distal ileum. There, control release was 0.06 ± 0.01 (GLP-1) and 0.07 ± 0.01 (PYY) pmol/cm2 of tissue. Rebaudioside A (2.5, 12.5, and 25 mM) stimulated GLP-1 release (0.14 ± 0.02, 0.16 ± 0.02, and 0.13 ± 0.02 pmol/cm2 of tissue, p < 0.001) and PYY release (0.19 ± 0.02, 0.42 ± 0.06, and 0.27 ± 0.03 pmol/cm2 of tissue, p < 0.001). Sucrose stimulated GLP-1 release (0.08 ± 0.01 pmol/cm2 of tissue, p < 0.05) only at 10 mM. Casein (0.5%, 1%, and 2.5%, w/v) stimulated GLP-1 release (0.15 ± 0.03, 0.13 ± 0.02, and 0.14 ± 0.01 pmol/cm2 of tissue, p < 0.001) and PYY release (0.13 ± 0.02, 0.20 ± 0.03, and 0.27 ± 0.03 pmol/cm2 of tissue, p < 0.01). These findings may help in developing dietary approaches for weight management. Subject LifeMSB - Microbiology and Systems BiologyELSS - Earth, Life and Social SciencesFood and NutritionNutritionHealthy LivingRebaudioside AGLP-1PYYEx vivo intestinal porcine modelCaseinPeptidesSugar (sucrose)Glucagon-like peptide-1Porcine modelsPYYSmall intestineSteviol glycosidesWeight managementTissue To reference this document use: http://resolver.tudelft.nl/uuid:df71c417-9985-435f-88b7-316cc8db134a DOI https://doi.org/10.1021/jf501105w TNO identifier 510985 Source Journal of Agricultural and Food Chemistry, 62 (33), 8365-8370 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.