Print Email Facebook Twitter Molecular characterization of trimellitic anhydride-induced respiratory allergy in Brown Norway rats Title Molecular characterization of trimellitic anhydride-induced respiratory allergy in Brown Norway rats Author Kuper, C.F. Heijne, W.H. Dansen, M. Verhoeckx, K.C. Boorsma, A. Radonjic, M. Bruijntjes, J. Stierum, R. Muijser, H. Arts, J.H. TNO Kwaliteit van Leven Publication year 2008 Abstract To contribute to the hazard identification of low molecular weight (LMW) respiratory allergens, respiratory allergy induced by trimellitic anhydride (TMA) was characterized by whole genome analysis of lung tissue and blood proteomics in Brown Norway rats. Dermal sensitization (50% and 25% w/v) with TMA and an inhalation challenge of 15 mg/m(3) TMA-induced apneas, laryngeal inflammation, increased numbers of eosinophils, neutrophils and macrophages in bronchoalveolar lavage (BAL), and increased immunoglobulin E levels in serum and lung tissue. Whole genome analysis of lung, sampled 24 hours after challenge, showed expression changes of not only genes belonging to several Gene Ontology groups with up-regulation of inflammatory-associated genes and those associated with lung remodeling but also genes involved in downsizing these processes. Blood proteomics reflected activation of inflammation-inhibiting pathways. Unsensitized animals challenged with TMA exhibited also an increased number of macrophages in BAL, but gene expression in the above-mentioned gene pathways was unchanged or down-regulated. The authors conclude that parameters for lung remodeling can be a valuable tool in hazard identification of LMW respiratory allergens. Subject BiologyToxicology and Applied PharmacologyAllergenImmunoglobulin EPhthalic anhydrideToll like receptorTrimellitic anhydrideAnalysis of varianceAnimalArticleBloodBrown Norway ratCluster analysisDNA microarrayDrug effectFemaleGene expression profilingGeneticsImmunohistochemistryLungLung lavageMetabolismNonparametric testPathologyPrincipal component analysisProteomicsRatRespiratory tract allergySignal transductionAllergensAnalysis of VarianceAnimalsBronchoalveolar LavageCluster AnalysisFemaleGene Expression ProfilingImmunoglobulin EImmunohistochemistryLungOligonucleotide Array Sequence AnalysisPhthalic AnhydridesPrincipal Component AnalysisProteomicsRatsRats, Inbred BNRespiratory HypersensitivitySignal TransductionStatistics, NonparametricToll-Like Receptors To reference this document use: http://resolver.tudelft.nl/uuid:ddcf1f44-ab39-4f6c-9644-22aebc3150ac DOI https://doi.org/10.1177/0192623308327410 TNO identifier 241243 ISSN 1533-1601 Source Toxicologic pathology, 36 (7), 985-998 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.