Print Email Facebook Twitter Spreads enriched with three different levels of vegetable oil sterols and the degree of cholesterol lowering in normocholesterolaemic and mildly hypercholesterolaemic subjects Title Spreads enriched with three different levels of vegetable oil sterols and the degree of cholesterol lowering in normocholesterolaemic and mildly hypercholesterolaemic subjects Author Hendriks, H.F.J. Weststrate, J.A. van Vliet, T. Meijer, G.W. Publication year 1999 Abstract Objective: To investigate the dose-response relationship between cholesterol lowering and three different, relatively low intake levels of plant sterols (0.83, 1.61, 3.24 g/d) from spreads. To investigate the effects on lipid-soluble (pro)vitamins. Design: A randomized double-blind placebo controlled balanced incomplete Latin square design using five spreads and four periods. The five study spreads included butter, a commercially available spread and three experimental spreads fortified with three different concentrations of plant sterols. Subjects: One hundred apparently healthy normocholesterolaemic and mildly hypercholesterolaemic volunteers participated. Interventions: Each subject consumed four spreads, each for a period of 3.5 week. Results: Compared to the control spread, total cholesterol decreased by 0.26 (CI: 0.15-0.36), 0.31 (CI: 0.20-0.41) and 0.35 (CI: 0.25-0.46) mmol/L, for daily consumption of 0.83, 1.61 and 3.24 g plant sterols, respectively. For LDL-cholesterol these decreases were 0.20 (CI: 0.10-0.31), 0.26 (CI: 0.15-0.36) and 0.30 (CI: 0.20-0.41). Decreases in the LDL/HDL ratio were 0.13 (CI: 0.04-0.22), 0.16 (CI: 0.07-0.24) and 0.16 (CI: 0.07-0.24) units, respectively. Differences in cholesterol reductions between the plant sterol doses consumed were not statistically significant. Plasma vitamin K1 and 25-OH-vitamin D and lipid standardized plasma lycopene and alpha-tocopherol were not affected by consumption of plant sterol enriched spreads, but lipid standardized plasma (alpha + beta)-carotene concentrations were decreased by about 11 and 19% by daily consumption of 0.83 and 3.24 g plant sterols in spread, respectively. Conclusions: The three relatively low dosages of plant sterols had a significant cholesterol lowering effect ranging from 4.9-6.8%, 6.7-9.9% and 6.5-7.9%, for total, LDL-cholesterol and the LDL/HDL cholesterol ratio, respectively, without substantially affecting lipid soluble (pro)vitamins. No significant differences in cholesterol lowering effect between the three dosages of plant sterols could be detected. This study would support that consumption of about 1.6 g of plant sterols per day will benefically affect plasma cholesterol concentrations without seriously affecting plasma carotenoid concentrations. Sponsorship: Unilever Research Vlaardingen, NL Subject Cholesterol-lowering dietDosageLipid soluble (pro)vitaminsPlant sterolsSpreadsAlpha caroteneAlpha tocopherolBeta caroteneCholesterolHigh density lipoprotein cholesterolLow density lipoprotein cholesterolLycopenePhytomenadioneDterolvegetable oilVitaminButterCholesterol blood levelClinical trialControlled clinical trialControlled studyFood intakeHuman experimentHypercholesterolemiaLatin square designLipid solubilityRandomized controlled trialStatistical analysisVitamin blood levelAdultAntioxidantsButterCarotenoidsCholesterolCholesterol, HDLCholesterol, LDLDose-Response Relationship, DrugDouble-Blind MethodFemaleHumansHypercholesterolemiaMaleMargarineMiddle AgedPhytosterolsQuestionnairesVitamin DVitamin EVitamin K To reference this document use: http://resolver.tudelft.nl/uuid:dcfebcb3-4aa0-4566-bd42-1601897a691e TNO identifier 86648 Source European Journal of Clinical Nutrition, 53, 319-327 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.