Print Email Facebook Twitter Bioavailability and biodistribution of differently charged polystyrene nanoparticles upon oral exposure in rats Title Bioavailability and biodistribution of differently charged polystyrene nanoparticles upon oral exposure in rats Author Walczak, A.P. Hendriksen, P.J.M. Woutersen, R.A. van der Zande, M. Undas, A.K. Helsdingen, R. van den Berg, H.H.J. Rietjens, I.M.C.M. Bouwmeester, H. Publication year 2015 Abstract The likelihood of oral exposure to nanoparticles (NPs) is increasing, and it is necessary to evaluate the oral bioavailability of NPs. In vitro approaches could help reducing animal studies, but validation against in vivo studies is essential. Previously, we assessed the translocation of 50 nm polystyrene NPs of different charges (neutral, positive and negative) using a Caco-2/HT29-MTX in vitro intestinal translocation model. The NPs translocated in a surface charge-dependent manner. The present study aimed to validate this in vitro intestinal model by an in vivo study. For this, rats were orally exposed to a single dose of these polystyrene NPs and the uptake in organs was determined. A negatively charged NP was taken up more than other NPs, with the highest amounts in kidney (37.4 µg/g tissue), heart (52.8 µg/g tissue), stomach wall (98.3 µg/g tissue) and small intestinal wall (94.4 µg/g tissue). This partly confirms our in vitro findings, where the same NPs translocated to the highest extent. The estimated bioavailability of different types of NPs ranged from 0.2 to 1.7 % in vivo, which was much lower than in vitro (1.6–12.3 %). Therefore, the integrated in vitro model cannot be used for a direct prediction of the bioavailability of orally administered NPs. However, the model can be used for prioritizing NPs before further in vivo testing for risk assessment. © 2015, The Author(s). Subject LifeRAPID - Risk Analysis for Products in DevelopmentELSS - Earth, Life and Social SciencesBiomedical InnovationNanotechnologyHealthy LivingBioavailabilityBiodistributionIn vivoOral exposurePolystyrene nanoparticlesSurface propertiesBiochemistryNanoparticlesPolystyrenesRatsRisk assessmentTissueNanoparticlePolystyreneAnimal experimentAnimal modelAnimal tissueControlled studyHeartIn vitro studyIn vivo studyIntestine wallKidneyMaleNonhumanPredictionSmall intestineStomach wallSurface charge To reference this document use: http://resolver.tudelft.nl/uuid:db61916d-717f-41b8-8290-c751b89da50c DOI https://doi.org/10.1007/s11051-015-3029-y TNO identifier 526114 ISSN 1388-0764 Source Journal of Nanoparticle Research, 17 (5) Article number 231 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.