Title
Identification of growth phenotype-related genes in Aspergillus oryzae by heterologous macroarray and suppression subtractive hybridization
Author
te Biesebeke, R.
Levin, A.
Sagt, C.
Bartels, J.
Goosen, T.
Ram, A.
van den Hondel, C.
Punt, P.
TNO Kwaliteit van Leven
Publication year
2005
Abstract
Aspergillus oryzae requires polarized growth for colonization of solid substrates, and this growth phenotype differs from that seen in liquid medium. Various experimental approaches were used to identify genes that are differentially expressed when A. oryzae is grown on wheat kernels and in a wheat-based liquid medium. Hybridization of A. oryzae RNAs to a macroarray bearing cDNAs isolated from a library representing at least 16% of the total number of A. niger genes identified 14 differentially expressed cDNA clones, showing that heterologous macroarray analysis with an A. niger cDNA library can be used to identify regulated gene transcripts in the related species A. oryzae. Moreover, Northern analysis with a selection of eight probes for A. niger genes encoding proteins involved in morphological development and cell wall biosynthesis identified five more differentially expressed genes. A suppression subtractive hybridization procedure revealed another 12 differentially expressed genes. The results presented show that, of the 29 identified genes which are expressed at higher levels during growth on wheat kernels, six encode proteins that are functionally related to polarized growth, four encode products known to be involved in morphogenesis, three code for proteins related to cell wall composition, and nine of the cDNA clones encode novel proteins. These findings pinpoint genes associated with the changes in cellular morphogenesis seen in A. oryzae grown on wheat kernels as opposed to wheat-based liquid medium. © Springer-Verlag 2005.
Subject
Biology
Biotechnology
Aspergillus
Heterologous hybridization
Macroarray hybridization
Morphology
Polarized growth
complementary DNA
RNA
article
Aspergillus niger
Aspergillus oryzae
biosynthesis
cell wall
controlled study
DNA isolation
DNA library
DNA probe
fungus growth
gene expression
genetic transcription
liquid culture
molecular cloning
morphogenesis
morphological trait
nonhuman
Northern blotting
nucleotide sequence
phenotype
priority journal
protein expression
seed kernel
suppression subtractive hybridization
wheat
Aspergillus oryzae
Base Sequence
Blotting, Northern
Computational Biology
DNA Primers
Gene Expression Regulation, Fungal
Gene Library
Genes, Fungal
Molecular Sequence Data
Nucleic Acid Hybridization
Phenotype
Sequence Analysis, DNA
Triticum
Aspergillus
Aspergillus niger
Aspergillus oryzae
Triticum aestivum
To reference this document use:
http://resolver.tudelft.nl/uuid:da1360c4-bdaa-4639-8eae-cf4c2b4d3c83
DOI
https://doi.org/10.1007/s00438-004-1082-9
TNO identifier
238360
ISSN
1617-4615
Source
Molecular Genetics and Genomics, 273 (1), 33-42
Document type
article