Title
Digestibility of resistant starch containing preparations using two in vitro models
Author
Fässler, C.
Arrigoni, E.
Venema, K.
Hafner, V.
Brouns, F.
Amadò, R.
TNO Kwaliteit van Leven
Publication year
2006
Abstract
Background: Resistant starch (RS) is known for potential health benefits in the human colon. To investigate these positive effects it is important to be able to predict the amount, and the structure of starch reaching the large intestine. Aim of the study: The aim of this study was to compare two different in vitro models simulating the digestibility of two RS containing preparations. Methods: The substrates, high amylose maize (HAM) containing RS type 2, and retrograded long chain tapioca maltodextrins (RTmd) containing RS type 3 were in vitro digested using a batch and a dynamic model, respectively. Both preparations were characterized before and after digestion by using X-Ray and DSC, and by measuring their total starch, RS and protein contents. Results: Using both digestion models, 60-61 g/100 g of RTmd turned out to be indigestible, which is very well in accordance with 59 g/100 g found in vivo after feeding RTmd to ileostomy patients. In contrast, dynamic and batch in vitro digestion experiments using HAM as a substrate led to 58 g/100 g and 66 g/100 g RS recovery. The degradability of HAM is more affected by differences in experimental parameters compared to RTmd. The main variations between the two in vitro digestion methods are the enzyme preparations used, incubation times and mechanical stress exerted on the substrate. However, for both preparations dynamically digested fractions led to lower amounts of analytically RS and a lower crystallinity. Conclusions: The two in vitro digestion methods used attacked the starch molecules differently, which influenced starch digestibility of HAM but not of RTmd. © 2006 Steinkopff Verlag Darmstadt.
Subject
Nutrition
Biomedical Research
DSC
In vitro digestion
Resistant starch type 2
Resistant starch type 3
X-ray
amylose
maltodextrin
resistant starch
starch
article
comparative study
concentration response
controlled study
crystal structure
differential scanning calorimetry
digestion
food analysis
human
ileostomy
in vitro study
incubation time
maize
mechanical stress
model
molecular dynamics
protein analysis
protein content
tapioca
X ray analysis
Amylose
Dietary Fiber
Digestion
Dose-Response Relationship, Drug
Polysaccharides
Starch
Substrate Specificity
Time Factors
Manihot esculenta
Zea mays
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http://resolver.tudelft.nl/uuid:d93379c9-eaef-4f8a-96e5-b53cd7621ada
DOI
https://doi.org/10.1007/s00394-006-0618-7
TNO identifier
239741
ISSN
1436-6207
Source
European Journal of Nutrition, 45 (8), 445-453
Document type
article