Print Email Facebook Twitter Butyrate enemas do not affect human colonic MUC2 and TFF3 expression Title Butyrate enemas do not affect human colonic MUC2 and TFF3 expression Author Hamer, H.M. Jonkers, D.M.A.E. Renes, I.B. Vanhoutvin, S.A.L.W. Kodde, A. Troost, F.J. Venema, K. Brummer, R.-J.M. TNO Kwaliteit van Leven Publication year 2010 Abstract INTRODUCTION: The colonic mucus layer plays an important role in the protection of the intestinal epithelium and mainly consists of mucin glycoproteins (primarily MUC2 in the colon) trefoil factor 3 (TFF3) and secretory IgA. Butyrate is a major end product of fermentation of dietary fibres and is associated with beneficial effects on colonic health. Earlier in-vitro and animal studies showed that butyrate modulates MUC2 and TFF3 expression and mucin secretion, although data from human studies are not yet available. Methods: Sixteen healthy volunteers and 35 ulcerative colitis (UC) patients in clinical remission self-administered a 60'ml rectal enema containing 100'mmol/l butyrate or placebo once daily for 2 and 3 weeks, respectively. After each treatment, biopsies were taken from the distal sigmoid for quantitative RT-PCR and immunohistochemical analysis of MUC2 and TFF3. In addition, mucosal sections were stained with high iron diamine-alcian blue to distinguish between sialomucins and sulphomucins. To analyse total mucin secretion and secretory IgA concentrations, 24'h faeces were collected during the day before the endoscopic examination. Results: The butyrate intervention did not significantly modulate the expression of MUC2 (fold change: 1.04 and 1.05 in healthy volunteers and ulcerative colitis patients, respectively) or TFF3 (fold change: 0.91 and 0.94 in healthy volunteers and UC patients, respectively). Furthermore, the percentage of sialomucins, mucus secretion and secretory IgA concentrations were not affected by the butyrate intervention in both the groups. Conclusion: Butyrate exposure in healthy volunteers and UC patients in remission did not affect the measured parameters of the colonic mucus layer. Copyright © 2010 Lippincott Williams & Wilkins. Subject BiologyBiomedical ResearchbutyrateMUC2mucinsecretory IgAtrefoil factorulcerative colitisalcian bluebutyric aciddiamineenemaintestinal trefoil factorintestinal trefoil factor 3mucin 2placebosecretory immunoglobulinsialomucinsulfomucinunclassified drugadultarticleclinical articleclinical trialcontrolled clinical trialcontrolled studycrossover proceduredrug effectfemalehistochemistryhumanhuman tissueimmunohistochemistryin vitro studymalenucleotide sequencepriority journalprotein expressionprotein secretionquantitative analysisrandomized controlled trialreal time polymerase chain reactionsigmoidtreatment durationulcerative colitis To reference this document use: http://resolver.tudelft.nl/uuid:d85087cc-850c-4d6d-85f0-22afdfd2875f TNO identifier 409256 ISSN 0954-691X Source European Journal of Gastroenterology and Hepatology, 22 (9), 1134-1140 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.