Title
Selection of Arabidopsis mutants overexpressing genes driven by the promoter of an auxin-inducible glutathione S-transferase gene
Author
van der Kop, D.A.M.
Schuyer, M.
Pinas, J.E.
van der Zaal, B.J.
Hooykaas, P.J.J.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
1999
Abstract
Transgenic arabidopsis plants were isolated that contained a T-DNA construct in which the promoter of an auxin-inducible glutathione S-transferase (GST) gene from tobacco was fused to the kanamycin resistance (nptII) as well as to the β-glucuronidase (gusA) reporter gene. Subsequently, seeds were treated with EMS to obtain mutants in which both reporter gene fusions were up-regulated. Northern analysis showed that the mRNA level of a related, endogenous auxin-inducible GST gene of Arabidopsis was increased in some of these mutants as well. Two of the gup (GST up-regulated) mutants were characterized in more detail and roughly mapped. Both had epinastic cotyledons and leaves, a phenotype that turned out to be linked to the gup mutation. Chemicals/CAS: DNA, Bacterial; Ethyl Methanesulfonate, 62-50-0; Glutathione Transferase, EC 2.5.1.18; Indoleacetic Acids; Mutagens; Recombinant Fusion Proteins; T-DNA
Subject
Nutrition
Arabidopsis thaliana
Auxin-responsive gene expression
Developmental mutants
Glutathione S-transferase
GST
Gup mutant
Auxin
Beta glucuronidase
Gene expression
Glutathione transferase
Kanamycin
Reporter gene
Transgenic plant
Arabidopsis
Chromosome Mapping
Crosses, Genetic
DNA, Bacterial
Enzyme Induction
Ethyl Methanesulfonate
Gene Expression Regulation, Plant
Genes, Plant
Glutathione Transferase
Indoleacetic Acids
Mutagenesis
Mutagens
Mutation
Phenotype
Plants, Genetically Modified
Promoter Regions (Genetics)
Recombinant Fusion Proteins
Up-Regulation
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DOI
https://doi.org/10.1023/a:1006129426712
TNO identifier
86824
ISSN
0167-4412
Source
Plant Molecular Biology, 39 (5), 979-990
Document type
article