Print Email Facebook Twitter Determination of alkylphosphonic acids by microcolumn liquid chromatography with gradient elution coupled on-line with flame photometric detection Title Determination of alkylphosphonic acids by microcolumn liquid chromatography with gradient elution coupled on-line with flame photometric detection Author Hooijschuur, E.W.J. Kientz, C.E. Brinkman, U.A.T. Publication year 2001 Abstract Microcolumn liquid chromatography with gradient elution and on-line flame photometric detection has been used for the selective and rapid determination of non-volatile alkylphosphonic acids, which are hydrolysis products of nerve agents, in aqueous samples. A make-up of water was used to maintain stable introduction of the eluent during a gradient run. The detection limits are strongly dependent on quenching, which is determined by the mass flow of methanol in the eluent. Large-volume injections of 100 μl of aqueous solutions of short- and long-chain alkylphosphonic acids resulted in detection limits in the range 6-800 ng/ml. The repeatability of the retention times and analyte response - peak area or peak height - were 0.7-0.9% and 4-11%, respectively. The method was successfully applied to a local tap water sample and an aqueous soil extract. © 2001 Elsevier Science B.V. Subject Alkylphosphonic acidsDetection, LCFlame photometric detectionLarge-volume injectionsWarfare agentsAlkyl groupPhosphonic acid derivativeChemical analysisChemical warfareFlame photometryHydrolysisLiquid chromatographyMolecular stabilityReproducibilityChromatography, LiquidPhosphonic AcidsPhotometry To reference this document use: http://resolver.tudelft.nl/uuid:d6187120-3303-44e5-96e5-439b912f2209 DOI https://doi.org/10.1016/s0021-9673(00)01005-0 TNO identifier 235952 Source Journal of Chromatography A, 907 (907), 165-172 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.