Title
Determination of alkylphosphonic acids by microcolumn liquid chromatography with gradient elution coupled on-line with flame photometric detection
Author
Hooijschuur, E.W.J.
Kientz, C.E.
Brinkman, U.A.T.
Publication year
2001
Abstract
Microcolumn liquid chromatography with gradient elution and on-line flame photometric detection has been used for the selective and rapid determination of non-volatile alkylphosphonic acids, which are hydrolysis products of nerve agents, in aqueous samples. A make-up of water was used to maintain stable introduction of the eluent during a gradient run. The detection limits are strongly dependent on quenching, which is determined by the mass flow of methanol in the eluent. Large-volume injections of 100 μl of aqueous solutions of short- and long-chain alkylphosphonic acids resulted in detection limits in the range 6-800 ng/ml. The repeatability of the retention times and analyte response - peak area or peak height - were 0.7-0.9% and 4-11%, respectively. The method was successfully applied to a local tap water sample and an aqueous soil extract. © 2001 Elsevier Science B.V.
Subject
Alkylphosphonic acids
Detection, LC
Flame photometric detection
Large-volume injections
Warfare agents
Alkyl group
Phosphonic acid derivative
Chemical analysis
Chemical warfare
Flame photometry
Hydrolysis
Liquid chromatography
Molecular stability
Reproducibility
Chromatography, Liquid
Phosphonic Acids
Photometry
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DOI
https://doi.org/10.1016/s0021-9673(00)01005-0
TNO identifier
235952
Source
Journal of Chromatography A, 907 (907), 165-172
Document type
article