Title
Comparability of microbiota of swabbed and spit saliva
Author
Kaan, A.M.
Brandt, B.W.
Buijs, M.J.
Crielaard, W.
Keijser, B.J.F.
Zaura, E.
Publication year
2022
Abstract
In general, saliva is used for microbiota analysis in longitudinal studies, and several collection methods are being used. Using a robust sample collection procedure is important, as it may influence salivary composition. This study explored the comparability of the microbiota of swabbed and spit saliva. Twenty-two females participated in this cross-sectional study. The bacterial composition of the three saliva samples (swab collected by the participant (SW-P), swab collected by the researcher (SW-R), and spit (SP) was assessed by 16S rRNA gene amplicon sequencing. The bacterial composition of the swabbed and the spit saliva was significantly different irrespective of the operator, and Shannon diversity was significantly higher in spit saliva than in SW-P and SW-R. The salivary microbiota of spit and swabbed adult saliva differs significantly. Research on microbial composition therefore requires collection of similar saliva sample types in all study participants. © 2021 The Authors. European Journal of Oral Sciences published by John Wiley & Sons Ltd on behalf of Scandinavian Division of the International Association for Dental Research.
Subject
16S rDNA
Microbiology
Oral
Silva
Microbiota
To reference this document use:
http://resolver.tudelft.nl/uuid:d5737adc-1dc1-41e5-b932-c325d36a2126
DOI
https://doi.org/10.1111/eos.12858
TNO identifier
965967
Source
European Journal of Oral Sciences, 130 (130), e12858
Document type
article