Print Email Facebook Twitter The isolated chicken eye test as a suitable in vitro method for determining the eye irritation potential of household cleaning products Title The isolated chicken eye test as a suitable in vitro method for determining the eye irritation potential of household cleaning products Author Schutte, K. Prinsen, M.K. McNamee, P.M. Roggeband, R. TNO Kwaliteit van Leven Publication year 2009 Abstract Eye irritation is an important endpoint in the safety evaluation of consumer products and their ingredients. Several in vitro methods have been developed and are used by different industry sectors to assess eye irritation. One such in vitro method in use for some time already is the isolated chicken eye test (ICE). This investigation focuses on assessing the ICE as a method to determine the eye irritation potential of household cleaning products, both for product safety assurance prior to marketing and for classification and labeling decisions. The ICE involves a single application of test substances onto the cornea of isolated chicken eyes. Endpoints are corneal swelling, corneal opacity and fluorescein retention. The ICE results were compared to historic LVET data in this study due to availability of such in vivo data and the ability to correlate LVET to human experience data on the outcome of accidental exposures to household cleaning products in general. The results of this study indicate that the ICE test is a useful in vitro method for evaluating the eye irritation/corrosion potential and establishing classification and labeling for household cleaning products. For new product formulations, it is best used as part of a weight-of-evidence approach and benchmarked against data from comparable formulations with known eye irritation/corrosion profiles and market experience. © 2009 Elsevier Inc. All rights reserved. Subject ToxicologyAlternative testing methodCEETChicken enucleated eye testEye irritationHousehold cleaning productsICEIn vitro test methodsIsolated chicken eye testLow volume eye testLVETcitric aciddetergentdomestic chemicalfluoresceinanimal tissuearticlechickenclassificationcontrolled studycorneacornea edemacornea opacitycorrosioneye irritationfemalein vitro studyindustryisolated organmalemarketingnonhumanpackagingpriority journalsafety To reference this document use: http://resolver.tudelft.nl/uuid:d555582d-724f-422e-af47-ecc480462727 DOI https://doi.org/10.1016/j.yrtph.2009.05.008 TNO identifier 241677 ISSN 0273-2300 Source Regulatory Toxicology and Pharmacology, 54 (3), 272-281 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.