Expression and production of llama variable heavy-chain antibody fragments (VHHs) by Aspergillus awamori
van den Hondel, C.A.M.J.J.
TNO Kwaliteit van Leven TNO Voeding
We report the expression and production of llama variable heavy-chain antibody fragments (VHHs) by Aspergillus awamori. Fragments encoding VHHs were cloned in a suitable Aspergillus expression vector and transformants secreting VHH fragments were analysed for integrated gene copy-numbers, mRNA levels and protein production. Functional V HHs were detected in the culture medium, indicating the feasibility of producing this type of protein in a fungal expression system. Secreted V HHs were subjected to (extracellular) degradation, which could be partially prevented by the addition of BSA to the culture medium. © Springer-Verlag 2004.
To reference this document use:
Heavy-chain antibody fragments
Amino Acid Sequence
Camelids, New World
Immunoglobulin Heavy Chains
Immunoglobulin Variable Region
Molecular Sequence Data
Sequence Homology, Amino Acid
Applied Microbiology and Biotechnology, 66 (4), 384-392