Title
Expression and production of llama variable heavy-chain antibody fragments (VHHs) by Aspergillus awamori
Author
Joosten, V.
Gouka, R.J.
van den Hondel, C.A.M.J.J.
Verrips, C.T.
Lokman, B.C.
TNO Kwaliteit van Leven TNO Voeding
Publication year
2005
Abstract
We report the expression and production of llama variable heavy-chain antibody fragments (VHHs) by Aspergillus awamori. Fragments encoding VHHs were cloned in a suitable Aspergillus expression vector and transformants secreting VHH fragments were analysed for integrated gene copy-numbers, mRNA levels and protein production. Functional V HHs were detected in the culture medium, indicating the feasibility of producing this type of protein in a fungal expression system. Secreted V HHs were subjected to (extracellular) degradation, which could be partially prevented by the addition of BSA to the culture medium. © Springer-Verlag 2004.
Subject
Biology
Biotechnology
Cloning
Degradation
Functions
Fungi
Proteins
RNA
Vanadium compounds
Aspergillus expression
Heavy-chain antibody fragments
mRNA levels
Antibodies
Antibody
Messenger RNA
Protein
Antibody
Antibody production
Aspergillus awamori
Culture medium
Expression vector
Extracellular space
Molecular cloning
Nonhuman
Nucleotide sequence
Protein degradation
Protein expression
Amino Acid Sequence
Animals
Aspergillus
Camelids, New World
Cloning, Molecular
Gene Expression
Immunoglobulin Heavy Chains
Immunoglobulin Variable Region
Molecular Sequence Data
Recombinant Proteins
Sequence Homology, Amino Acid
Transformation, Genetic
Aspergillus
Aspergillus awamori
Lama glama
vectors
To reference this document use:
http://resolver.tudelft.nl/uuid:d4d3e519-39ba-456a-8a07-39ea018f70ad
DOI
https://doi.org/10.1007/s00253-004-1689-0
TNO identifier
238288
ISSN
0175-7598
Source
Applied Microbiology and Biotechnology, 66 (4), 384-392
Document type
article