Title
Expression of cbsA encoding the collagen-binding S-protein of Lactobacillus crispatus JCM5810 in Lactobacillus casei ATCC 393T
Author
Martínez, B.
Sillanpää, J.
Smit, E.
Korhonen, T.K.
Pouwels, P.H.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
2000
Abstract
The cbsA gene encoding the collagen-binding S-layer protein of Lactobacillus crispatus JCM5810 was expressed in L. casei ATCC 393T. The S-protein was not retained on the surface of the recombinant bacteria but was secreted into the medium. By translational fusion of CbsA to the cell wall sorting signal of the proteinase, PrtP, of L. casei, CbsA was presented at the surface, rendering the transformants able to bind to immobilized collagens. Chemicals/CAS: Bacterial Proteins; Integrins; Membrane Glycoproteins; Membrane Proteins; Receptors, Collagen; surface array protein, bacteria
Subject
Nutrition
Bacterial protein
Binding protein
Collagen binding s protein
Unclassified drug
Bacterium adherence
Gene expression
Lactobacillus
Lactobacillus casei
Lactobacillus crispatus
Nonhuman
Priority journal
Protein secretion
Signal transduction
Bacterial Proteins
Cell Membrane
Integrins
Lactobacillus
Lactobacillus casei
Membrane Glycoproteins
Membrane Proteins
Receptors, Collagen
Bacteria (microorganisms)
Lactobacillus casei
Lactobacillus crispatus
Posibacteria
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DOI
https://doi.org/10.1128/jb.182.23.6857-6861.2000
TNO identifier
41891
ISSN
0021-9193
Source
Journal of Bacteriology, 182 (23), 6857-6861
Document type
article