Print Email Facebook Twitter Expression of cbsA encoding the collagen-binding S-protein of Lactobacillus crispatus JCM5810 in Lactobacillus casei ATCC 393T Title Expression of cbsA encoding the collagen-binding S-protein of Lactobacillus crispatus JCM5810 in Lactobacillus casei ATCC 393T Author Martínez, B. Sillanpää, J. Smit, E. Korhonen, T.K. Pouwels, P.H. Centraal Instituut voor Voedingsonderzoek TNO Publication year 2000 Abstract The cbsA gene encoding the collagen-binding S-layer protein of Lactobacillus crispatus JCM5810 was expressed in L. casei ATCC 393T. The S-protein was not retained on the surface of the recombinant bacteria but was secreted into the medium. By translational fusion of CbsA to the cell wall sorting signal of the proteinase, PrtP, of L. casei, CbsA was presented at the surface, rendering the transformants able to bind to immobilized collagens. Chemicals/CAS: Bacterial Proteins; Integrins; Membrane Glycoproteins; Membrane Proteins; Receptors, Collagen; surface array protein, bacteria Subject NutritionBacterial proteinBinding proteinCollagen binding s proteinUnclassified drugBacterium adherenceGene expressionLactobacillusLactobacillus caseiLactobacillus crispatusNonhumanPriority journalProtein secretionSignal transductionBacterial ProteinsCell MembraneIntegrinsLactobacillusLactobacillus caseiMembrane GlycoproteinsMembrane ProteinsReceptors, CollagenBacteria (microorganisms)Lactobacillus caseiLactobacillus crispatusPosibacteria To reference this document use: http://resolver.tudelft.nl/uuid:d49c3efc-0002-40d8-ba19-e6b7e7fc9cc3 DOI https://doi.org/10.1128/jb.182.23.6857-6861.2000 TNO identifier 41891 ISSN 0021-9193 Source Journal of Bacteriology, 182 (23), 6857-6861 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.