Title
Differences in dexamethasone-sensitivity between lymphocytes from patients with Alzheimer's disease and patients with multi-infarct dementia
Author
Nijhuis, E.W.P.
Oostervink, F.
Hinloopen, B.
Rozing, J.
Nagelkerken, L.
Gaubius Instituut TNO
Publication year
1996
Abstract
Peripheral blood mononuclear cells (PBMC) from 40 consecutive patients entering a screening program on cognitive impairment were studied in vitro with respect to their sensitivity to dexamethasone (DEX). Phytohemagglutinin-induced proliferation by PBMC from patients with senile dementia of the Alzheimer type (SDAT) was less sensitive to the inhibitory effect of DEX, compared to PBMC from patients with multi-infarct dementia (MID) and PBMC from patients with miscellaneous causes of cognitive impairment (MISC). An intermediate sensitivity was found with PBMC from patients with clinical signs of both MID and SDAT (= MIXED). These differences could not be explained by differences in the composition of the CD4+ T cell population, interleukin (IL)-2 or IL-4 production, or quantitative differences in the expression of glucocorticoid receptors as measured by flowcytometry. However, the expression of bcl-2 was higher in PBMC from SDAT patients than in cells from MID patients or from MISC patients, whereas the MIXED group showed an intermediate expression; a high bcl-2 expression correlated with a low DEX-sensitivity. These findings suggest that characteristics of PBMC reflect related changes in the central nervous system and indicate that PBMC may be a useful and accessible tool to obtain more insight into the pathogenesis of Alzheimer's disease. Chemicals/CAS: dexamethasone receptor; Dexamethasone, 50-02-2; Immunosuppressive Agents; Interleukin-2; Interleukin-4, 207137-56-2; Phytohemagglutinins; Proto-Oncogene Proteins c-bcl-2; Receptors, Glucocorticoid
Subject
Health
Glucocorticoid receptor
Clinical article
Cognitive defect
Drug sensitivity
Human cell
Lymphocyte
Mononuclear cell
Multiinfarct dementia
Screening
Aged
Aged, 80 and over
Alzheimer Disease
Apoptosis
Cells, Cultured
Dementia, Multi-Infarct
Dexamethasone
Drug Resistance
Female
Gene Expression Regulation
Humans
Immunosuppressive Agents
Interleukin-2
Interleukin-4
Lymphocyte Activation
Male
Phytohemagglutinins
Proto-Oncogene Proteins c-bcl-2
Receptors, Glucocorticoid
T-Lymphocytes
To reference this document use:
http://resolver.tudelft.nl/uuid:d33229fc-c018-4833-abd3-79124ddbb484
DOI
https://doi.org/10.1006/brbi.1996.0012
TNO identifier
233352
ISSN
0889-1591
Source
Brain, Behavior, and Immunity, 10 (2), 115-125
Document type
article