Title
The contact allergen dinitrochlorobenzene (DNCB) and respiratory allergy in the Th2-prone Brown Norway rat
Author
Kuper, C.F.
Stierum, R.H.
Boorsma, A.
Schijf, M.A.
Prinsen, M.
Bruijntjes, J.P.
Bloksma, N.
Arts, J.H.E.
TNO Kwaliteit van Leven
Publication year
2008
Abstract
All LMW respiratory allergens known to date can also induce skin allergy in test animals. The question here was if in turn skin allergens can induce allergy in the respiratory tract. Respiratory allergy was tested in Th2-prone Brown Norway (BN) rats by dermal sensitization with the contact allergen dinitrochlorobenzene (DNCB; 1%, day 0; 0.5%, day 7) and a head/nose-only inhalation challenge of 27 mg/m3 of DNCB (15 min, day 21), using a protocol that successfully identified chemical respiratory allergens. Skin allergy to DNCB was examined in BN rats and Th1-prone Wistar rats in a local lymph node assay followed by a topical patch challenge of 0.1% DNCB. Sensitization of BN rats via the skin induced DNCB-specific IgG in serum, but not in all animals, and an increased number of CD4+ cells in the lung parenchyma. Subsequent inhalation challenge with DNCB did not provoke apneas or allergic inflammation (signs of respiratory allergy) in the BN rats. However, microarray analysis of mRNA isolated from the lung revealed upregulation of the genes for Ccl2 (MCP-1), Ccl4 (MIP-1beta), Ccl7 and Ccl17. Skin challenge induced considerably less skin irritation and allergic dermatitis in the BN rat than in the Wistar rat. In conclusion, the Th2-prone BN rat appeared less sensitive to DNCB than the Wistar rat; nevertheless, DNCB induced allergic inflammation in the skin of BN rats but even a relatively high challenge concentration did not induce allergy in the respiratory tract, although genes associated with allergy were upregulated in lung tissue. © 2008 Elsevier Ireland Ltd. All rights reserved.
Subject
Health
Dinitrochlorobenzene
LLNA
MCP-1
MIP1-beta
Respiratory allergy
Skin allergy
1 chloro 2,4 dinitrobenzene
CD4 antigen
immunoglobulin G
macrophage inflammatory protein 1beta
monocyte chemotactic protein 1
monocyte chemotactic protein 3
thymus and activation regulated chemokine
animal experiment
animal model
animal tissue
article
controlled study
female
lung function
lung lavage
lung parenchyma
male
microarray analysis
nonhuman
Norway
nucleotide sequence
priority journal
rat
respiratory tract allergy
skin allergy
skin irritation
skin sensitization
Th2 cell
upregulation
Administration, Cutaneous
Allergens
Animals
Dermatitis, Allergic Contact
Dinitrochlorobenzene
Female
Immunoglobulin E
Immunoglobulin G
Inhalation Exposure
Irritants
Local Lymph Node Assay
Lung
Lymph Nodes
Male
Rats
Rats, Inbred BN
Rats, Wistar
Respiratory Function Tests
Respiratory Hypersensitivity
Th2 Cells
Animalia
Rattus
Rattus norvegicus
To reference this document use:
http://resolver.tudelft.nl/uuid:d2dceb6c-9403-4b69-b63c-8b20f93f721a
DOI
https://doi.org/10.1016/j.tox.2008.01.013
TNO identifier
240761
ISSN
0300-483X
Source
Toxicology, 246 (2-3), 213-221
Document type
article