Print Email Facebook Twitter Low Dose Betaine Supplementation Leads to Immediate and Long Term Lowering of Plasma Homocysteine in Healthy Men and Women Title Low Dose Betaine Supplementation Leads to Immediate and Long Term Lowering of Plasma Homocysteine in Healthy Men and Women Author Olthof, M.R. van Vliet, T. Boelsma, E. Verhoef, P. Publication year 2003 Abstract High plasma homocysteine is a risk for cardiovascular disease and can be lowered through supplementation with 6 g/d of betaine. However, dietary intake of betaine is ∼0.5-2 g/d. Therefore, we investigated whether betaine supplementation in the range of dietary intake lowers plasma homocysteine concentrations in healthy adults. Four groups of 19 healthy subjects ingested three doses of betaine or placebo daily for 6 wk. A methionine loading test was performed during run in, on d 1 of betaine supplementation, and after 2 and 6 wk of betaine supplementation. Fasting plasma homocysteine after 6-wk daily intakes of 1.5, 3 and 6 g of betaine was 12% (P < 0.01), 15% (P < 0.002) and 20% (P < 0.0001) less than in the placebo group, respectively. Furthermore, the increase in plasma homocysteine after methionine loading on the 1st d of betaine supplementation was 16% (P < 0.06), 23% (P < 0.008) and 35% (P < 0.0002) less than in the placebo group, respectively, and after 6 wk of supplementation was 23% (P < 0.02), 30% (P < 0.003) and 40% (P < 0.0002) less, respectively. Thus, doses of betaine in the range of dietary intake reduce fasting and postmethionine loading plasma homocysteine concentrations. A betaine-rich diet might therefore lower cardiovascular disease risk. Subject Health BiologyPhysiological SciencesBetaineHomocysteineHumanInterventionMethioninebetainehomocysteinelactosemethionineplaceboadultamino acid blood levelcardiovascular diseaseclinical trialconference papercontrolled clinical trialcontrolled studydiet supplementationdietary intakefemalehigh performance liquid chromatographyhumanhyperhomocysteinemiamalenormal humanAdultBetaineDietary SupplementsDose-Response Relationship, DrugDouble-Blind MethodFastingFemaleHomocysteineHumansMaleMethioninePlacebosReference Values To reference this document use: http://resolver.tudelft.nl/uuid:d28ff61a-4eba-40b2-af1c-0c32caaa5476 TNO identifier 237443 ISSN 0022-3166 Source Journal of Nutrition, 133 (12), 4135-4138 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.