Title
Analysis of temporal gene expression during Bacillus subtilis spore germination and outgrowth
Author
Keijser, B.J.F.
ter Beek, A.
Rauwerda, H.
Schuren, F.
Montijn, R.
van der Spek, H.
Brul, S.
TNO Kwaliteit van Leven
Publication year
2007
Abstract
Bacillus subtilis forms dormant spores upon nutrient depletion. Under favorable environmental conditions, the spore breaks its dormancy and resumes growth in a process called spore germination and outgrowth. To elucidate the physiological processes that occur during the transition of the dormant spore to an actively growing vegetative cell, we studied this process in a time-dependent manner by a combination of microscopy, analysis of extracellular metabolites, and a genome-wide analysis of transcription. The results indicate the presence of abundant levels of late sporulation transcripts in dormant spores. In addition, the results suggest the existence of a complex and well-regulated spore outgrowth program, involving the temporal expression of at least 30% of the B. subtilis genome. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Subject
Biology
Biotechnology
Cell division
Cell growth
DNA repair
DNA replication
Microscopy
Spore germination
Sporogenesis
Transcription regulation
Bacillus subtilis
Gene Expression
Gene Expression Profiling
Oligonucleotide Array Sequence Analysis
RNA, Bacterial
RNA, Messenger
Spores, Bacterial
Time Factors
Transcription, Genetic
Bacillus subtilis
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http://resolver.tudelft.nl/uuid:d2849b7d-da38-4f13-9136-3a993bf91cea
DOI
https://doi.org/10.1128/jb.01736-06
TNO identifier
239955
ISSN
0021-9193
Source
Journal of Bacteriology, 189 (9), 3624-3634
Document type
article