Title
Alkaloid profiling of the Chinese herbal medicine Fuzi by combination of matrix-assisted laser desorption ionization mass spectrometry with liquid chromatography-mass spectrometry
Author
Wang, J.
van der Heijden, R.
Spijksma, G.
Reijmers, T.
Wang, M.
Xu, G.
Hankemeier, T.
van der Greef, J.
TNO Kwaliteit van Leven
Publication year
2009
Abstract
A matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) method was developed for the high throughput and robust qualitative profiling of alkaloids in Fuzi-the processed lateral roots of the Chinese herbal medicine Aconitum carmichaeli Debx (A. carmichaeli). After optimization, powdered roots - without any further sample preparation - could be used to screen for the presence of Aconitum alkaloids. Furthermore, the semi-quantitative potential of MALDI-MS was confirmed using liquid chromatography-mass spectrometry (LC-MS) as reference. In total over sixty alkaloids were detected by LC-MS and fifteen of them were tentatively identified. Both MALDI-MS and LC-MS analysis revealed significant variation in alkaloid content in different (commercial) samples. LC-MS analysis of three toxic alkaloids in 14 batches of Fuzi resulted in a variation of their concentrations expressed as RSDs of 138%, 99% and 221% for aconitine, hypaconitine and mesaconitine, respectively. The variation in concentrations (expressed as RSD) of about the ninety constituents detected were classified as follows: 13 constituents showed an RSD of 77-100%, 46 with an RSD of 100-150%, 21 with an RSD of 150-200% and 9 constituents with an RSD in concentration of 200-235%. These results demonstrate a strong difference in chemical composition of the various Fuzi and illustrate the necessity of adequate QA/QC procedures for both safety and efficiency of herbal medicine. The described analytical procedures for alkaloid profiling could play a role in these procedures. © 2008 Elsevier B.V. All rights reserved.
Subject
Health
Analytical research
Biomedical research
Aconitum carmichaeli Debx
Alkaloid profiling
Herbal medicine
LC-MS
MALDI-MS
Aconitum carmichaeli Debx
Alkaloid profiling
Herbal medicine
LC-MS
MALDI-MS
Body fluids
Chromatographic analysis
Chromatography
Desorption
Ionization
Ionization of liquids
Liquid chromatography
Mass spectrometers
Mass spectrometry
Medicine
Semiconductor switches
Spectrometers
Spectrometry
Spectrum analysis
High performance liquid chromatography
Aconitum extract
Aconitine
Aconitum
Alkaloids
Chromatography, Liquid
Drugs, Chinese Herbal
Linear Models
Mass Spectrometry
Plant Roots
Quality Control
Reproducibility of Results
Sensitivity and Specificity
Aconitum
Aconitum carmichaeli
To reference this document use:
http://resolver.tudelft.nl/uuid:d0b22e6d-9142-4471-9e93-221106593c8a
DOI
https://doi.org/10.1016/j.chroma.2008.11.077
TNO identifier
241440
ISSN
0021-9673
Source
Journal of Chromatography A, 1216 (11), 2169-2178
Document type
article