Print Email Facebook Twitter Screening and confirmation criteria for hormone residue analysis using liquid chromatography accurate mass time-of-flight, Fourier transform ion cyclotron resonance and orbitrap mass spectrometry techniques Title Screening and confirmation criteria for hormone residue analysis using liquid chromatography accurate mass time-of-flight, Fourier transform ion cyclotron resonance and orbitrap mass spectrometry techniques Author Nielen, M.W.F. van Engelen, M.C. Zuiderent, R. Ramaker, R. TNO Kwaliteit van Leven Publication year 2007 Abstract An emerging trend is recognised in hormone and veterinary drug residue analysis from liquid chromatography tandem mass spectrometry (LC/MS/MS) based screening and confirmation towards accurate mass alternatives such as LC coupled with time-of-flight (TOF), Fourier transform ion cyclotron resonance (FTICR) or Fourier transform orbitrap (FT Orbitrap) MS. In this study, mass resolution and accuracy are discussed for LC/MS screening and confirmation of targeted analytes and for the identification of unknowns using the anabolic steroid stanozolol and the designer β-agonist "Clenbuterol-R" as model substances. It is shown theoretically and experimentally that mass accuracy criteria without proper mass resolution criteria yield false compliant (false negative) results, both in MS screening and MS/MS confirmation of stanozolol. On the other hand, previous medium resolution accurate mass TOFMS/MS data of the designer β-agonist were fully confirmed by high resolution FT Orbitrap MSn experiments. A discussion is initiated through a proposal for additional criteria for the use of accurate mass LC/MS technologies, to be implemented in Commission Decision 2002/657/EC. © 2006 Elsevier B.V. All rights reserved. Subject BiologyAnalytical researchAccurate massCriteriaFourier transform mass spectrometryLiquid chromatography mass spectrometryOrbitrapResidue analysisSteroidCyclotron resonanceData reductionLiquid chromatographyMass spectrometryVeterinary medicineAccurate massFourier transform mass spectrometryOrbitrapResidue analysisSteroidHormonesanabolic agentbeta adrenergic receptor stimulating agentclenbuterolhormonestanozololaccuracyarticleconceptual frameworkfalse negative resultidentification keyion cyclotron resonance mass spectrometryliquid chromatographymass spectrometrypriority journalresidue analysisscreeningsex hormone determinationtime of flight mass spectrometryAnimalsChemistry, AnalyticalChromatography, LiquidClenbuterolCyclotronsDrug ResiduesFourier AnalysisIonsMass SpectrometryModels, ChemicalReproducibility of ResultsSensitivity and SpecificitySpectrometry, Mass, Electrospray IonizationStanozololSteroids To reference this document use: http://resolver.tudelft.nl/uuid:cc33ba97-8340-4cef-b43d-c198e5f3d738 DOI https://doi.org/10.1016/j.aca.2006.08.055 TNO identifier 239897 ISSN 0003-2670 Source Analytica Chimica Acta, 586 (1-2 SPEC. ISS.), 122-129 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.