Title
Using in vitro/in silico data for consumer safety assessment of feed flavoring additives e A feasibility study using piperine
Author
Thiel, A.
Etheve, S.
Fabian, E.
Leeman, W.R.
Plautz, J.R.
Publication year
2015
Abstract
Consumer health risk assessment for feed additives is based on the estimated human exposure to the additive that may occur in livestock edible tissues compared to its hazard.We present an approach using alternative methods for consumer health risk assessment. The aim was to use the fewest possible number of animals to estimate its hazard and human exposure without jeopardizing the safety upon use. As an example we selected the feed flavoring substance piperine and applied in silico modeling for residue estimation, results from literature surveys, and Read-Across to assess metabolism in different species. Results were compared to experimental in vitro metabolism data in rat and chicken, and to quantitative analysis of residues' levels from the in vivo situation in livestock. In silico residue modeling showed to be a worst case: the modeled residual levels were considerably higher than the measured residual levels. The in vitro evaluation of livestock versus rodent metabolism revealed no major differences in metabolism between the species.We successfully performed a consumer health risk assessment without performing additional animal experiments. As shown, the use and combination of different alternative methods supports animal welfare consideration and provides future perspective to reducing the number of animals.
Subject
Life
RAPID - Risk Analysis for Products in Development
ELSS - Earth, Life and Social Sciences
Food and Nutrition
Nutrition
Healthy Living
Residue estimation
Transfer database
Piperine
Metabolism
Risk assessment
To reference this document use:
http://resolver.tudelft.nl/uuid:c7680b94-2701-4355-83aa-d5b092ca4c56
DOI
https://doi.org/10.1016/j.yrtph.2015.06.006
TNO identifier
526357
Source
Regulatory Toxicology and Pharmacology, 73, 73-84
Document type
article