Pulmonary response of hamsters to fibrous glass. - Clearance and morphology after a single intratracheal instillation
van Graft, M.
Instituut CIVO-Toxicologie en voeding TNO
In a one-year serial sacrifice study 10 mg glass fibres (length, 95% ?? 20 ??m, 89% ?? 12 ??m, 58% ?? 5 ??m and 25% ?? 2 ??m; diameter, 88% ?? 1.0 ??m, 60% ?? 0.5 ??m and 31% ??0.25 ??m) suspended in 0.2 ml saline solution were administered to Syrian golden hamsters by a single intratracheal instillation to determine the clearance of the glass fibres from the lungs and to examine their effects on the lungs using light and electron microscopy. The clearance was rather efficient with a half-time of about 3 months. Coating and corrosion of glass fibres were sporadic findings. A violent focal acute pneumonitis was evoked by the glass fibres and was followed by excessive accumulations of alveolar macrophages often loaden with glass fibres. Thereafter, 'silicotic granulomas' developed which were seen as clusters of tightly packed iron-positive macrophages containing glass fibres. These granulomas were surrounded by a layer of alveolar epithelium, and were by the end of the study the predominant lesion in the otherwise normal lungs. Chemicals/CAS: fiberglass; Glass
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Experimental Pathology, 29 (4), 197-209