Title
Production and characterization of a monoclonal antibody reactive with a specific neoantigenic determinant (comprising B?? 54-118) in degradation products of fibrin and of fibrinogen
Author
Koppert, P.W.
Koopman, J.
Haverkate, F.
Nieuwenhuizen, W.
Gaubius instituut TNO
Publication year
1986
Abstract
Balb/c mice were immunized with a mixture of fibrin degradation products (XDPs) prepared by complete lysis of a human bloot clot by tissue-type plasminogen activator and purified by immunoaffinity chromatography. Spleen cells of the mice were fused with P3 x 63 Ag 8653 myeloma cells. A clone (FDP 14) was selected that produces monoclonal antibodies (MoAbs) of the IgG1?? type that react with a neoantigenic determinant exposed in these XDPs, but not in intact fibrinogen or in fibrin monomers. Furthermore, the MoAb is reactive with some pure, individual degradation products of fibrinogen (fragments X, Y, E, and the N-terminal disulphide knot) and with the fibrinogen B??-chain but not with A??- and ??-chains or with fragments D, FCB-2 and FCB-3. Comparison of the known primary structures of these fibrinogen fragments indicates that the stretch B?? 54-118 comprises at least an important part of the epitope recognized by FDP-14. Apparently, this stretch contributes importantly to a neoantigenic determinant that is not functional in intact fibrinogen and fibrin monomer and that can be made functional by reduction of fibrinogen, or by digestion with plasmin or CNBr. Chemicals/CAS: fibrin, 9001-31-4; fibrinogen, 9001-32-5; Antibodies, Monoclonal; Fibrin Fibrinogen Degradation Products; Fibrin, 9001-31-4; Fibrinogen, 9001-32-5
Subject
Monoclonal antibody
Blood and hemopoietic system
Enzyme linked immunosorbent assay
Fibrinogen b beta chain
Fibrinolysis
Mouse
Neoantigen
Nonhuman
Animal
Antibodies, Monoclonal
Antibody Formation
Antibody Specificity
Antigen-Antibody Reactions
Female
Fibrin
Fibrin Fibrinogen Degradation Products
Fibrinogen
Human
Mice
Mice, Inbred BALB C
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TNO identifier
230047
ISSN
0006-4971
Source
Blood, 68 (2), 437-441
Document type
article