Title
In vitro proliferation of haemopoietic cells in the presence of adherent cell layers. II. Differential effect of adherent cell layers derived from various organs
Author
Reimann, J.
Burger, H.
Radiobiologisch Instituut TNO
Publication year
1979
Abstract
Mouse bone marrow-derived adherent cell populations promoted proliferation of haemopoietic cells in vitro in a liquid culture system for at least 4 weeks. Adherent cell layers derived from other haemopoietic organs (foetal liver, adult spleen) and fibroblasts from embryonic tissues did not maintain haemopoietic cells in this system. Medium, conditioned by adherent cells derived from foetal liver, spleen and embryonic tissues, displayed a considerable colony stimulating activity (CSA). Marrow-derived adherent cells produced no detectable CSA. A possible relationship between the in vitro expression of a growth-promoting activity of an adherent cell population on haemopoietic cells, and its endogenous CSA production, is discussed. Chemicals/CAS: Culture Media
Subject
Animal
Bone Marrow Cells
Cell Division
Cells, Cultured
Colony-Forming Units Assay
Culture Media
Fibroblasts
Hematopoietic Stem Cells
Liver
Mice
Mice, Inbred BALB C
Spleen
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TNO identifier
228771
ISSN
0301-472X
Source
Experimental Hematology, 7 (1), 52-58
Document type
article