Title
Detection of outdoor mould staining as biofinish on oil treated wood
Author
van Nieuwenhuijzen, E.J.
Sailer, M.F.
Gobakken, L.R.
Adan, O.C.G.
Punt, P.J.
Samson, R.A.
Publication year
2015
Abstract
Stains on wood are often unwanted in outdoor applications, dark stain formation however is essential to the development of a new protective, self-healing and decorative biotreatment for wood. The biotreatment is based on the formation of surface covering mould staining on linseed oil treated pine sapwood during outdoor exposure. This specific stain formation is called biofinish and an assessment method is proposed in this study. Analysis of the visual stain coverage and quantification of the darkness generated useful input for the detection of biofinishes. Analysis of the microbial composition of a biofinish by PCR amplification and (Sanger) sequencing of fungal DNA sequences was appropriate for identification of Aureobasidium, as the dominant genus in several biofinishes, however our results indicate, that a more in depth, next generation sequencing method is preferred for a more elaborate biofinish assessment method. With the basic assessment method, biofinish formation was determined for oil treated wood specimens exposed outdoors in the Netherlands and some selected sites outside the Netherlands. Biofinish formation was demonstrated to be reproducible for pine sapwood, which was treated with raw linseed oil and exposed in the Netherlands. Furthermore olive oil is discovered in this study as a supportive factor for biofinish formation, regardless of the wood type, whereas biofinish formation was not detected on wood treated with stand linseed oil. © 2015 Elsevier Ltd.
Subject
Life
MSB - Microbiology and Systems Biology
ELSS - Earth, Life and Social Sciences
Biomedical Innovation
Biology
Healthy Living
Aureobasidium
Biofilm
Linseed oil
Sustainable
Wood protection
Wood staining
Biofilms
DNA sequences
Drying oils
Gene encoding
Molds
Oilseeds
Olive oil
Polymerase chain reaction
Wood products
Microbial composition
Next-generation sequencing
PCR amplification
Surface covering
Wood
To reference this document use:
http://resolver.tudelft.nl/uuid:c0c083ce-2ce7-413f-9494-ea45252d3a36
DOI
https://doi.org/10.1016/j.ibiod.2015.09.001
TNO identifier
528366
ISSN
0964-8305
Source
International Biodeterioration and Biodegradation, 105, 215-227
Article number
3742
Document type
article