Print Email Facebook Twitter Metabolomics of prolonged fasting in humans reveals new catabolic markers Title Metabolomics of prolonged fasting in humans reveals new catabolic markers Author Rubio-Aliaga, I. de Roos, B. Duthie, S.J. Crosley, L.K. Mayer, C. Horgan, G. Colquhoun, I.J. Le Gall, G. Huber, F. Kremer, W. Rychlik, M. Wopereis, S. van Ommen, B. Schmidt, G. Heim, C. Bouwman, F.G. Mariman, E.C. Mulholland, F. Johnson, I.T. Polley, A.C. Elliott, R.M. Daniel, H. Centraal Instituut voor Voedingsonderzoek TNO Publication year 2011 Abstract Fasting is one of the simplest metabolic challenges that can be performed in humans. We here report for the first time a comprehensive analysis of the human "fasting metabolome" obtained from analysis of plasma and urine samples in a small cohort of healthy volunteers, using nuclear magnetic resonance (NMR), gas chromatography- and liquid chromatography-mass spectrometry (GC-MS and LC-MS). Intra- and inter-individual variation of metabolites was on measurement of four overnight fasting samples collected from each volunteer over a four week period. One additional sample per volunteer was collected following a prolonged fasting period of 36 h. Amongst a total of 377 quantified entities in plasma around 44% were shown to change significantly in concentration when volunteers extended fasting from 12 to 36 h. In addition to known markers (plasma free fatty acids, glycerol, ketone bodies) that reflect changes in the body's fuel management under fasting conditions a wide range of "new" entities such as a-aminobutyrate as well as other amino and keto acids were identified as fasting markers. Based on multiple correlations amongst the metabolites and selected hormones in plasma such as leptin or insulin-like-growth-factor-1 (IGF-1), a robust metabolic network with coherent regulation of a wide range of metabolites could be identified. The metabolomics approach described here demonstrates the plasticity of human metabolism and identifies new and robust markers of the fasting state. © 2010 Springer Science+Business Media, LLC. Chemicals/CAS: 2 aminobutyric acid, 80-60-4; glycerol, 56-81-5; somatomedin C, 67763-96-6 Subject LifeMSB - Microbiology and Systems BiologyEELS - Earth, Environmental and Life SciencesNutrition BiologyFasting responseMetabolite profilingSubject variability To reference this document use: http://resolver.tudelft.nl/uuid:c08586bd-75f3-4972-bd87-b894534c8ddc DOI https://doi.org/10.1007/s11306-010-0255-2 TNO identifier 436009 ISSN 1573-3882 Source Metabolomics, 7 (3), 375-387 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.