Title
Enhanced urinary gelatinase activities (matrix metalloproteinases 2 and 9) are associated with early-stage bladder carcinoma: A comparison with clinically used tumor markers
Author
Sier, C.F.M.
Casetta, G.
Verheijen, J.H.
Tizzani, A.
Agape, V.
Kos, J.
Blasi, F.
Hanemaaijer, R.
Gaubius Instituut TNO
Publication year
2000
Abstract
Matrix metalloproteinases (MMPs) are involved in tumor growth and metastasis, promoting the migration and invasion of cells. In this study, the amount of MMP-2 and MMP-9 activity was measured in urine from superficial bladder carcinoma patients (pTa, pT1) to evaluate their possible diagnostic value. The active and total amount of MMP-2 and MMP-9, respectively, in urine from tumor patients were compared with the levels in urine from age and gender-matched healthy volunteers. Both MMP-2 and MMP-9 activity levels were significantly enhanced in urine from patients with high invasive cancers (pT2, PT3), whereas in urine from healthy controls no or very low MMP activities were found. More importantly, a substantial number of urine samples from patients with superficial tumors contained elevated MMP-2 and MMP-9 activities, suggesting that enhanced urinary MMP activity levels, indeed, might be indicative for early-stage bladder cancer. Overall, urinary MMP-2 and MMP-9 activity levels were significantly correlated to each other, with some individual exceptions. A comparison between urinary MMP-9 activity and a recently proposed urinary marker for bladder cancer, NMP-22, showed slightly lower numbers of patients with elevated levels for MMP-9. But because MMP-9 and NMP-22 levels were not correlated, enhanced urinary MMP activity might be useful as a marker for superficial bladder carcinoma like, or especially in combination with, other markers.
Subject
Adult
Aged
Aged, 80 and over
Carcinoma
Case-Control Studies
Cathepsin B
Creatinine
Enzyme-Linked Immunosorbent Assay
Female
Humans
Male
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Middle Aged
Neoplasm Invasiveness
Nuclear Proteins
Tumor Markers, Biological
Urinary Bladder Neoplasms
Urinary Plasminogen Activator
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TNO identifier
235565
ISSN
1078-0432
Source
Clinical Cancer Research, 6 (6), 2333-2340
Document type
article