Print Email Facebook Twitter Enhanced urinary gelatinase activities (matrix metalloproteinases 2 and 9) are associated with early-stage bladder carcinoma: A comparison with clinically used tumor markers Title Enhanced urinary gelatinase activities (matrix metalloproteinases 2 and 9) are associated with early-stage bladder carcinoma: A comparison with clinically used tumor markers Author Sier, C.F.M. Casetta, G. Verheijen, J.H. Tizzani, A. Agape, V. Kos, J. Blasi, F. Hanemaaijer, R. Gaubius Instituut TNO Publication year 2000 Abstract Matrix metalloproteinases (MMPs) are involved in tumor growth and metastasis, promoting the migration and invasion of cells. In this study, the amount of MMP-2 and MMP-9 activity was measured in urine from superficial bladder carcinoma patients (pTa, pT1) to evaluate their possible diagnostic value. The active and total amount of MMP-2 and MMP-9, respectively, in urine from tumor patients were compared with the levels in urine from age and gender-matched healthy volunteers. Both MMP-2 and MMP-9 activity levels were significantly enhanced in urine from patients with high invasive cancers (pT2, PT3), whereas in urine from healthy controls no or very low MMP activities were found. More importantly, a substantial number of urine samples from patients with superficial tumors contained elevated MMP-2 and MMP-9 activities, suggesting that enhanced urinary MMP activity levels, indeed, might be indicative for early-stage bladder cancer. Overall, urinary MMP-2 and MMP-9 activity levels were significantly correlated to each other, with some individual exceptions. A comparison between urinary MMP-9 activity and a recently proposed urinary marker for bladder cancer, NMP-22, showed slightly lower numbers of patients with elevated levels for MMP-9. But because MMP-9 and NMP-22 levels were not correlated, enhanced urinary MMP activity might be useful as a marker for superficial bladder carcinoma like, or especially in combination with, other markers. Subject AdultAgedAged, 80 and overCarcinomaCase-Control StudiesCathepsin BCreatinineEnzyme-Linked Immunosorbent AssayFemaleHumansMaleMatrix Metalloproteinase 2Matrix Metalloproteinase 9Middle AgedNeoplasm InvasivenessNuclear ProteinsTumor Markers, BiologicalUrinary Bladder NeoplasmsUrinary Plasminogen Activator To reference this document use: http://resolver.tudelft.nl/uuid:c031cc9e-c902-4f37-81dc-288e4c6548dc TNO identifier 235565 ISSN 1078-0432 Source Clinical Cancer Research, 6 (6), 2333-2340 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.