Print Email Facebook Twitter Urine profile analysis by field desorption mass spectrometry, a technique for detecting metabolites of xenobiotics. Application to 3,5-dinitro-2-hydroxytoluene Title Urine profile analysis by field desorption mass spectrometry, a technique for detecting metabolites of xenobiotics. Application to 3,5-dinitro-2-hydroxytoluene Author van der Greef, J. Leegwater, D.C. Publication year 1983 Abstract A technique for the detection of biotransformation products of xenobiotics in crude urine extracts by field desorption mass spectrometric profile analysis is described. The method comprises determination of peak profiles of a series of blank and test samples using low resolution field desorption mass spectrometry, comparison of averaged peak profiles and noise reduction by means of Fisher and ratio weighting of peak intensities. Application of the technique to 3,5-dinitro-2-hydroxytoluene has resulted in the detection of two hitherto unknown metabolites in rat urine. By thin-layer co-chromatography, high resolution electron impact mass spectrometry and thin-layer chromatographic-field desorption mass spectrometric analysis they could be identified as 3,5-dinitro-2-hydroxybenzenemethanol and 3,5-diacetamido-2-hydroxytoluene. Chemicals/CAS: dinitro ortho cresol, 534-52-1; Cresols; Dinitrocresols; hedolit, 534-52-1 Subject NutritionDinitro ortho cresolDrug metaboliteRadioisotopeXenobiotic agentDinitro ortho cresol c 14Drug analysisDrug determinationMass spectrometryMethodologyNonhumanThin layer chromatographyUrineAnimalBiotransformationCresolsDinitrocresolsMaleRatsRats, Inbred StrainsSpectrum Analysis, Mass To reference this document use: http://resolver.tudelft.nl/uuid:bf487f06-7572-4483-b62d-e51972cf149b TNO identifier 229378 ISSN 0306-042X Source Biomedical Mass Spectrometry, 10 (1), 1-4 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.