Print Email Facebook Twitter Children with peanut allergy recognize predominantly Ara h2 and Ara h6, which remains stable over time Title Children with peanut allergy recognize predominantly Ara h2 and Ara h6, which remains stable over time Author Flinterman, A.E. van Hoffen, E. den Hartog Jager, C.F. Koppelman, S. Pasmans, S.G. Hoekstra, M.O. Bruijnzeel-Koomen, C.A. Knulst, A.C. Knol, E.F. TNO Kwaliteit van Leven Publication year 2007 Abstract Background: In peanut-allergic adults, IgE is mainly directed to Ara h1 and Ara h2. More recently, a role for Ara h6 has been suggested. In contrast to adults, IgE in children can fluctuate over time. Therefore, children may have a more dynamic reactivity to peanut. Objective: To examine the IgE reactivity to major peanut allergens in peanut-allergic children at two subsequent time-points. Methods: Twenty children (3-15 years old) with peanut allergy, confirmed by a double-blind placebo-controlled food challenge (DBPCFC), were included. Just before and 20 months after DBPCFC, IgE reactivity to purified Ara h1, Ara h2, Ara h3 and Ara h6 was studied by immunoblots and skin prick tests (SPTs). Results: Before DBPCFC, all peanut-allergic children showed IgE reactivity to Ara h2; Ara h6 was recognized by 16 children, and Ara h1 and Ara h3 by 10 children. After 20 months, peanut-specific IgE levels (median 23 kU/L) and the individual recognition of major allergens were comparable with the levels and recognition before challenge (median 28.2 kU/L). SPT with Ara h2 and Ara h6 was positive in most children, whereas SPT with Ara h1 and Ara h3 was positive in approximately half of the children. Ara h6 induced the largest weals. None of the parameters were related to the severity of peanut allergy. Conclusion: Ara h2 and Ara h6 are the most frequently recognized major peanut allergens in children. The individual reactivity to the major peanut allergens remained stable over time, despite DBPCFC. © 2007 Blackwell Publishing Ltd. Subject NutritionFood technologyAra h2 and Ara h6ChildrenIgE reactivityPeanut allergyStable individual recognitionimmunoglobulin Eadolescentantigen recognitionarticlechildclinical articlecontrolled studyfemalehumanimmunoblottingimmunoreactivitymalepeanut allergyprick testpriority journalprovocation testskin testAdolescentAdultAllergensAntibody SpecificityChildChild, PreschoolDouble-Blind MethodFemaleGlycoproteinsHumansImmunoblottingImmunoglobulin EMalePeanut HypersensitivityPlant ProteinsSkin TestsTime Factors To reference this document use: http://resolver.tudelft.nl/uuid:be85f0b5-3648-4a57-bd13-e8279c2445ac DOI https://doi.org/10.1111/j.1365-2222.2007.02764.x TNO identifier 240102 ISSN 0954-7894 Source Clinical and Experimental Allergy, 37 (8), 1221-1228 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.