Title
Brown Norway rat ovalbumin-specific immunoglobulin E antibodies increase the human basophil expression of CD63 marker
Author
Bellou, A.
Saint-Laudy, J.
Knippels, L.
Montémont, C.
Vauthier, E.
Gerard, P.
Pellegrom, H.
Koerkamp, E.K.
Lesesve, J.F.
Guéant, J.L.
Lambert, H.
Mallié, J.P.
TNO Voeding
Publication year
2003
Abstract
Anaphylactic shock is an immunoglobulin E (IgE)-dependent hypersensitivity. Biological tests like leucocyte histamine release (LHR) and human basophil activation (HBA), frequently used in human allergy, reflect both the amount of IgE fixed on cells and the cellular reactivity. To assess whether serum-specific IgE from Brown Norway (BN) rats prepared for ovalbumin (OVA)-induced anaphylactic shocks can activate human basophils which has a potential interest in experimental allergy: such a test could rapidly assert an IgE sensitization in laboratory animals genetically T-helper 2 (Th2)-predisposed. Rats (n = 39) were immunized three times (day 0, day 5 and day 21) with OVA injected subcutaneously. One week after the third immunization, a shock was induced with an intravenous (i.v.) bolus of OVA. Sensitization was assessed by passive cutaneous anaphylaxis (PCA) test and dosages of serum IgE antibodies anti-OVA by enzyme-linked immunosorbent assay. Blood basophils were counted before and during the shock. Before the shock induction (at day 21), an LHR test was performed on rat blood, and human basophils were sensitized with rat sera. HBA was demonstrated by the increase in the percentage of cells expressing CD63 antigen membrane, measured by flow cytometry. Twenty-one days after the first subcutaneous (s.c.) immunization, the rat serum induced a significant HBA. HBA was observed neither with the same serum previously heated nor with the serum from nonimmunized rats (NIRs). OVA-specific IgEs were significantly increased in immunized rat (IR) serum. The PCA test was negative when the serum was previously heated (56°C). We never observed any circulating basophils, and LHR test was negative. After OVA i.v. administration, all IRs died rapidly. HBA testing strongly suggests a mediation by specific IgE in the increase of CD63 in BN rats. Thus, HBA test seems useful in assessing whether an experimental allergy was induced in animals genetically predisposed to an immune response, Th2-mediated, like BN rat. We also conclude that rat basophil activation does not participate in the histamine release during anaphylactic shock in sensitized BN rats.
Subject
Toxicology Biology
Toxicology and Applied Pharmacology
CD63 antigen
immunoglobulin E
ovalbumin
adolescent
allergy test
anaphylactic shock
animal model
antibody response
antibody specificity
antigen expression
article
basophil
cell activation
enzyme linked immunosorbent assay
histamine release
human
human cell
immunization
nonhuman
passive skin anaphylaxis
priority journal
rat
rat strain
sensitization
serum
Th2 cell
Anaphylaxis
Animals
Antigens, CD
Basophils
Enzyme-Linked Immunosorbent Assay
Histamine Release
Humans
Immunization
Immunoglobulin E
Ovalbumin
Passive Cutaneous Anaphylaxis
Platelet Membrane Glycoproteins
Rats
Rats, Inbred BN
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DOI
https://doi.org/10.1046/j.1365-3083.2003.01233.x
TNO identifier
236989
ISSN
0300-9475
Source
Scandinavian Journal of Immunology, 57 (3), 271-278
Document type
article