Title
Expression profiling of colon cancer cell lines and colon biopsies: Towards a screening system for potential cancer-preventive compounds
Author
van Erk, M.J.
Krul, C.A.M.
Caldenhoven, E.
Stierum, R.H.
Peters, W.H.
Woutersen, R.A.
van Ommen, B.
TNO Kwaliteit van Leven TNO Voeding
Publication year
2005
Abstract
Interest in mechanisms of colon cancer prevention by food compounds is strong and research in this area is often performed with cultured colon cancer cells. In order to assess utility for screening of potential cancer-preventive (food) compounds, expression profiles of 14 human cell lines derived from colonic tissue were measured using cDNA microarrays with 4000 genes and compared with expression profiles in biopsies of human colon tumours and normal tissue. Differences and similarities in the gene expression profiles of the cell lines were analysed by clustering and principal component analysis (PCA). Cytoskeleton genes and immune response genes are two functional classes of genes that contributed to the differences between the cell lines. A subset of 72 colon cancer-specific genes was identified by comparing expression profiles in human colon biopsies of tumour tissue and normal tissue. A separation of the cell lines based on the tumour stage of the original adenocarcinoma was observed after PCA of expression data of the subset of colon cancer-specific genes in the cell lines. The results of this study may be useful in the ongoing research into mechanisms of cancer prevention by dietary components. © 2005 Lippincott Williams & Wilkins.
Subject
Health
Toxicology and Applied Pharmacology
Cancer prevention
Cell lines
Colon biopsies
Colon cancer
Expression profiling
APC protein
Beta catenin
Complementary DNA
Cyclooxygenase 2
Protein p53
Adult
Cancer cell culture
Cancer prevention
Cancer research
Cancer screening
Cancer staging
Clinical article
Colon biopsy
Colon cancer
Controlled study
Data analysis
DNA microarray
Female
Gene expression
Genetic analysis
Human
Human cell
Hybridization
Immune response gene
Male
Mutation
Nucleotide sequence
Oncogene c myc
Oncogene ras
Principal component analysis
Priority journal
Protein expression
RNA isolation
Tumor suppressor gene
Adenocarcinoma
Adult
Biopsy
Cell Line, Tumor
Colonic Neoplasms
Female
Gastric Mucosa
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Genes, Neoplasm
Genetic Screening
Humans
Male
Middle Aged
Mutation, Missense
Neoplasm Proteins
Oligonucleotide Array Sequence Analysis
RNA, Neoplasm
Tumor Markers, Biological
To reference this document use:
http://resolver.tudelft.nl/uuid:bde4484d-12c7-4687-9c44-0907df3df7cd
DOI
https://doi.org/10.1097/01.cej.0000174781.51883.21
TNO identifier
238734
ISSN
0959-8278
Source
European Journal of Cancer Prevention, 14 (5), 439-457
Document type
article