Title
Effect of eugenol on the mutagenicity of benzo[a]pyrene and the formation of benzo[a]pyrene-DNA adducts in the X-lacZ-transgenic mouse.
Author
Rompelberg, C.J.M.
Steenwinkel, M.J.S.T.
van Asten, J.G.
van Delft, J.H.M.
Baan, R.A.
Verhagen, H.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
1996
Abstract
To study the possible reduction by eugenol of the mutagenicity and genotoxicity of benzoja]pyrene (B[a]P) in vivo, the X-lacZ-transgenic mouse strain 40.6 (Muta(TM)Mouse) was used. Male mice were fed a diet containing 0.4% (w/w) eugenol or a control diet for 58 days. On day 10, half of the mice received an i.p. dose of 100 mg/kg b.w. B[a]P. The lacZ mutants were recovered by packaging of DNA isolated from liver into lambda phage, and expressed in E. coli C lacZ- recA-galE- bacteria. In both control mice and mice fed the eugenol diet, B[a]P treatment resulted in a similar, significant increase in lacZ mutant frequency. Eugenol was not mutagenic by itself By 32P-postlabelling analysis of the liver DNA using an analysis method with chromatographic conditions for B[a]P-DNA adducts, no effect of eugenol on the formation of B[a]P-DNA adducts in the λ-lacZ-transgenic mouse was found. By 32P-postlabelling analysis using an alkenylbenzene solvent system the amount of B[a]P-DNA adducts was lower in mice fed the eugenol diet than in mice fed the control diet but the decrease was not statistically significant. However, one spot indicative of an eugenol-associated DNA adduct was detected. The present data provide no evidence for antimutagenic or antigenotoxic potential of eugenol in vivo Furthermore, they suggest genotoxicity in vivo of eugenol per se.
Subject
Toxicology
Antimutagenicity
Benzo[a]pyrene
DNA adduct
Eugenol
Transgenic mouse
benzo[a]pyrene
eugenol
animal experiment
article
controlled study
dna adduct
genotoxicity
male
mouse
mutagenicity
mutation rate
nonhuman
oral drug administration
priority journal
transgenic mouse
Animals
Antimutagenic Agents
Benzo(a)pyrene
Biotransformation
Body Weight
Diet
DNA Adducts
Eugenol
Glutathione Transferase
Lac Operon
Liver
Male
Mice
Mice, Transgenic
Mutagens
Phosphorus Radioisotopes
Animalia
Gale
Mus musculus
To reference this document use:
http://resolver.tudelft.nl/uuid:bd305644-0948-4afa-a7ad-5bb75aa73def
DOI
https://doi.org/10.1016/s0165-1218(96)90052-x
TNO identifier
233398
ISSN
0165-1218
Source
Mutation Research - Genetic Toxicology, 369 (1), 87-96
Document type
article