Print Email Facebook Twitter Effect of eugenol on the mutagenicity of benzo[a]pyrene and the formation of benzo[a]pyrene-DNA adducts in the X-lacZ-transgenic mouse. Title Effect of eugenol on the mutagenicity of benzo[a]pyrene and the formation of benzo[a]pyrene-DNA adducts in the X-lacZ-transgenic mouse. Author Rompelberg, C.J.M. Steenwinkel, M.J.S.T. van Asten, J.G. van Delft, J.H.M. Baan, R.A. Verhagen, H. Centraal Instituut voor Voedingsonderzoek TNO Publication year 1996 Abstract To study the possible reduction by eugenol of the mutagenicity and genotoxicity of benzoja]pyrene (B[a]P) in vivo, the X-lacZ-transgenic mouse strain 40.6 (Muta(TM)Mouse) was used. Male mice were fed a diet containing 0.4% (w/w) eugenol or a control diet for 58 days. On day 10, half of the mice received an i.p. dose of 100 mg/kg b.w. B[a]P. The lacZ mutants were recovered by packaging of DNA isolated from liver into lambda phage, and expressed in E. coli C lacZ- recA-galE- bacteria. In both control mice and mice fed the eugenol diet, B[a]P treatment resulted in a similar, significant increase in lacZ mutant frequency. Eugenol was not mutagenic by itself By 32P-postlabelling analysis of the liver DNA using an analysis method with chromatographic conditions for B[a]P-DNA adducts, no effect of eugenol on the formation of B[a]P-DNA adducts in the λ-lacZ-transgenic mouse was found. By 32P-postlabelling analysis using an alkenylbenzene solvent system the amount of B[a]P-DNA adducts was lower in mice fed the eugenol diet than in mice fed the control diet but the decrease was not statistically significant. However, one spot indicative of an eugenol-associated DNA adduct was detected. The present data provide no evidence for antimutagenic or antigenotoxic potential of eugenol in vivo Furthermore, they suggest genotoxicity in vivo of eugenol per se. Subject ToxicologyAntimutagenicityBenzo[a]pyreneDNA adductEugenolTransgenic mousebenzo[a]pyreneeugenolanimal experimentarticlecontrolled studydna adductgenotoxicitymalemousemutagenicitymutation ratenonhumanoral drug administrationpriority journaltransgenic mouseAnimalsAntimutagenic AgentsBenzo(a)pyreneBiotransformationBody WeightDietDNA AdductsEugenolGlutathione TransferaseLac OperonLiverMaleMiceMice, TransgenicMutagensPhosphorus RadioisotopesAnimaliaGaleMus musculus To reference this document use: http://resolver.tudelft.nl/uuid:bd305644-0948-4afa-a7ad-5bb75aa73def DOI https://doi.org/10.1016/s0165-1218(96)90052-x TNO identifier 233398 ISSN 0165-1218 Source Mutation Research - Genetic Toxicology, 369 (1), 87-96 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.