Title
Intragastric immunization with recombinant Lactobacillus casei expressing flagellar antigen confers antibody-independent protective immunity against Salmonella enterica serovar Enteritidis
Author
Kajikawa, A.
Satoh, E.
Leer, R.J.
Yamamoto, S.
Igimi, S.
TNO Kwaliteit van Leven
Publication year
2007
Abstract
A recombinant Lactobacillus casei expressing a flagellar antigen from Salmonella enterica serovar Enteritidis was constructed and evaluated as a mucosal vaccine. Intragastric immunization of the recombinant strain conferred protective immunity against Salmonella infection in mice. This immunization did not result in antigen-specific antibody in either feces or sera but induced the release of IFN-γ on restimulation of primed lymphocytes ex vivo. The results suggested that the protective efficacy provided by flagellin-expressing L. casei is mainly attributable to cell-mediated immune responses. In addition, an adjuvant-type effect of the antigen delivery system with L. casei was also observed. © 2007 Elsevier Ltd. All rights reserved.
Subject
Biology
Biotechnology
Flagellin
Lactobacillus
Salmonella
Vaccine
antibody
antigen
flagellin
gamma interferon
recombinant antigen
animal experiment
animal model
animal tissue
antibody specificity
antigen antibody complex
antigen expression
antigen specificity
article
bacterial infection
bacterial strain
cellular immunity
drug efficacy
ex vivo study
feces analysis
female
immune response
immunity
immunization
Lactobacillus casei
lymphocyte
mouse
nonhuman
priority journal
Salmonella enterica
Salmonella enteritidis
salmonellosis
serum
Administration, Oral
Animals
Female
Flagellin
Humans
Immunization
Interferon Type II
Lactobacillus casei
Lymphocyte Activation
Mice
Mice, Inbred C3H
Recombination, Genetic
Salmonella enteritidis
Salmonella Infections, Animal
Salmonella Vaccines
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DOI
https://doi.org/10.1016/j.vaccine.2007.01.055
TNO identifier
239976
ISSN
0264-410X
Source
Vaccine, 25 (18), 3599-3605
Document type
article