Print Email Facebook Twitter Ileal brake activation: Macronutrient-specific effects on eating behavior? Title Ileal brake activation: Macronutrient-specific effects on eating behavior? Author van Avesaat, M. Troost, F.J. Ripken, D. Hendriks, H.F. Aam, M. Publication year 2015 Abstract BACKGROUND: Activation of the ileal brake, by infusing lipid directly into the distal part of the small intestine, alters gastrointestinal (GI) motility and inhibits food intake. The ileal brake effect on eating behavior of the other macronutrients is currently unknown. OBJECTIVE: The objective of this study was to investigate the effects of ileal infusion of sucrose and casein on food intake, release of GI peptides, gastric emptying rate and small-bowel transit time with safflower oil as positive control. DESIGN: This randomized, single-blind, crossover study was performed in 13 healthy subjects (6 male; mean age 26.4 ± 2.9 years; mean body mass index 22.8 ± 0.4 kgm-2) who were intubated with a naso-ileal catheter. Thirty minutes after the intake of a standardized breakfast, participants received an ileal infusion, containing control ((C) saline), safflower oil ((HL) 51.7 kcal), low-dose casein ((LP) 17.2 kcal) or high-dose casein ((HP) 51.7 kcal), low-dose sucrose ((LC) 17.2 kcal) and high-dose sucrose ((HC) 51.7 kcal), over a period of 90 min. Food intake was determined during an ad libitum meal. Visual analogue score questionnaires for hunger and satiety and blood samples were collected at regular intervals. RESULTS: Ileal infusion of lipid, protein and carbohydrate resulted in a significant reduction in food intake compared with control (HL: 464.3 ± 90.7 kcal, P < 0.001; HP: 458.0 ± 78.6 kcal, P < 0.005; HC: 399.0 ± 57.0 kcal, P < 0.0001 vs control: 586.7 ± 70.2 kcal, P < 0.001, respectively). A reduction in energy intake was still apparent when the caloric amount of infused nutrients was added to the amount eaten during the ad libitum meal. Secretion of cholecystokinin and peptide YY but not of glucagon-like peptide-1 (7-36) was increased during ileal perfusion of fat, carbohydrates and protein. During ileal perfusion of all macronutrients, a delay in gastric emptying and intestinal transit was observed, but differences were not significant compared with control. CONCLUSION: Apart from lipids, also sucrose and casein reduce food intake on ileal infusion, thereby activating the ileal brake. In addition to food intake, also satiety and GI peptide secretion were affected. Chemicals/CAS: casein, 9000-71-9; cholecystokinin, 9011-97-6, 93443-27-7; glucagon like peptide 1, 89750-14-1; peptide YY, 81858-94-8; safflower oil, 8001-23-8; sucrose, 122880-25-5, 57-50-1 Subject LifeMSB - Microbiology and Systems BiologyELSS - Earth, Life and Social SciencesHealthy for LifeHealthHealthy LivingCaseinCholecystokininGastrointestinal polypeptideGlucagon like peptide 1Peptide YYPlaceboSafflower oilSucroseAdultCaloric intakeControlled studyCrossover procedureFeeding behaviorFemaleFood intakeHumanHuman experimentIleum infusionInfusionIntestine transit timeMacronutrientMaleNormal humanPriority journalProtein secretionRandomized controlled trialSingle blind procedureStomach emptyingCarthamus tinctoriusNaso To reference this document use: http://resolver.tudelft.nl/uuid:bafaafd4-36b4-4a4c-9e10-94e9ac7f639c DOI https://doi.org/10.1038/ijo.2014.112 TNO identifier 523204 ISSN 0307-0565 Source International Journal of Obesity, 39 (2), 235-243 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.