Title
Changes in the oral ecosystem induced by the use of 8% arginine toothpaste
Author
Koopman, J.E.
Hoogenkamp, M.A.
Buijs, M.J.
Brandt, B.W.
Keijser, B.J.F.
Crielaard, W.
ten Cate, J.M.
Zaura, E.
Publication year
2017
Abstract
Objective Bacterial metabolism of arginine in the oral cavity has a pH-raising and thus, potential anti-caries effect. However, the influence of arginine on the oral microbial ecosystem remains largely unresolved. Design In this pilot study, nine healthy individuals used toothpaste containing 8% arginine for eight weeks. Saliva was collected to determine arginolytic potential and sucrose metabolic activity at the Baseline, Week 4, Week 8 and after a two weeks Wash-out period. To follow the effects on microbial ecology, 16S rDNA sequencing on saliva and plaque samples at Baseline and Week 8 and metagenome sequencing on selected saliva samples of the same time-points was performed. Results During the study period, the arginolytic potential of saliva increased, while the sucrose metabolism in saliva decreased. These effects were reversed during the Wash-out period. Although a few operational taxonomic units (OTUs) in plaque changed in abundance during the study period, there was no real shift in the plaque microbiome. In the saliva microbiome there was a significant compositional shift, specifically the genus Veillonella had increased significantly in abundance at Week 8. Conclusion Indeed, the presence of arginine in toothpaste affects the arginolytic capacity of saliva and reduces its sucrose metabolic activity. Additionally, it leads to a shift in the salivary microbiome composition towards a healthy ecology from a caries point of view. Therefore, arginine can be regarded as a genuine oral prebiotic. © 2016 Elsevier Ltd
Subject
Life
MSB - Microbiology and Systems Biology
ELSS - Earth, Life and Social Sciences
Biomedical Innovation
Biology
Healthy Living
Arginine
Microbiome
Oral ecology
Prebiotic
To reference this document use:
http://resolver.tudelft.nl/uuid:badacf4c-7a48-41e5-9258-4054b0784089
DOI
https://doi.org/10.1016/j.archoralbio.2016.09.008
TNO identifier
572911
ISSN
0003-9969
Source
Archives of Oral Biology, 73, 79-87
Bibliographical note
Funding Details: Colgate-Palmolive Company
Document type
article