Title
Age-related decrease in proteoglycan synthesis of human articular chondrocytes: The role of nonenzymatic glycation
Author
de Groot, J.
Verzijl, N.
Bank, R.A.
Lafeber, F.P.J.G.
Bijlsma, J.W.J.
TeKoppele, J.M.
Publication year
1999
Abstract
Objective. To examine the effect of nonenzymatic glycation of cartilage extracellular matrix on the synthetic activity of chondrocytes. Methods. The proteoglycan-synthesis rate (35SO42- incorporation) and levels of advanced nonenzymatic glycation (determined by high-performance liquid chromatography measurement of pentosidine) were evaluated in human articular cartilage from 129 donors, varying in age from 25 to 88 years, and in cartilage with enhanced levels of advanced glycation end-products (AGEs) resulting from incubation with ribose. Results. Cartilage showed a strong age-related increase in pentosidine levels (r = 0.97, P < 0.0005) and, concomitantly, a decrease in proteoglycan synthesis (r = -0.98, P < 0.0002). This decrease in proteoglycan synthesis correlated with the increase in pentosidine (r = -0.95, P < 0.02). Moreover, the elevation of pentosidine levels in the in vitro-ribosylated cartilage was proportional with the decrease in proteoglycan synthesis (r = -0.95, P < 0.005). Conclusion. In both aged and in vitro AGE-enriched cartilage, the rate of proteoglycan synthesis was negatively correlated with the degree of glycation. This suggests that the age-related increase in cartilage AGE levels may be responsible, at least in part, for the age-related decline in the synthetic capacity of cartilage.
Subject
Health Biology
Biomedical Research
age
article
articular cartilage
cartilage cell
glycation
human
human tissue
pathogenesis
priority journal
proteoglycan synthesis
rheumatic disease
Adult
Aged
Aged, 80 and over
Aging
Arginine
Cartilage, Articular
Cross-Linking Reagents
Extracellular Matrix
Glycosylation End Products, Advanced
Humans
Lysine
Middle Aged
Osteoarthritis
Proteoglycans
Ribose
To reference this document use:
http://resolver.tudelft.nl/uuid:ba1f44be-88be-4a6c-b446-3d3744c186bd
DOI
https://doi.org/10.1002/1529-0131(199905)42:5<1003::aid-anr20>3.0.co;2-k
TNO identifier
235021
ISSN
0004-3591
Source
Arthritis and Rheumatism, 42 (5), 1003-1009
Document type
article