Title
Expression of receptors for gut peptides in pancreata of BOP-treated and control hamsters
Author
Tang, C.
Biemond, I.
Appel, M.J.
Visser, C.J.T.
Woutersen, R.A.
Lamers, C.B.H.W.
TNO Voeding
Publication year
1996
Abstract
The growth of pancreatic cancers may be influenced by certain gut peptides. However, the alteration of gut peptide receptors in the progress of pancreatic carcinogenesis is largely unknown. With storage phosphor autoradiography, this study visualized and characterized receptors for cholecystokinin (CCK), somatostatin (SST), bombesin (BBS), secretin and vasoactive intestinal peptide (VIP) in pancreata of control hamsters (n = 7) and pancreatic preneoplastic lesions (n = 10) or adenocarcinomas (n = 10) of N-nitrosobis(2-oxopropyl)amine (BOP)-treated hamsters. The specific CCK-A and secretin receptors expressed in normal pancreata were markedly reduced in pancreatic preneoplastic lesions and absent in adenocarcinomas. In the development of pancreatic tumours, the subgroup of SST receptors did not change, but both the affinity and binding capacity declined. In comparison with the binding of VIP to normal pancreata, specific VIP binding was significantly lower in preneoplastic lesions and almost absent in pancreatic adenocarcinomas. No specific binding for BBS was detected in normal pancreas or (pre)neoplastic lesions of hamster pancreas. The reduction or absence of receptors for CCK, secretin, SST and VIP in hamster pancreas with the progress of carcinogenesis suggests that in BOP-treated hamsters, pancreatic adenocarcinomas have, to a large extent, lost the hormone-dependent characteristics of the original tissue.
Subject
Biology
Bis(2 oxopropyl)nitrosamine
Bombesin receptor
Cholecystokinin receptor
Hormone receptor
Somatostatin
Somatostatin receptor
Vasoactive intestinal polypeptide receptor
Animal experiment
Animal tissue
Controlled study
Hamster
Hormone receptor interaction
Nonhuman
Pancreas adenocarcinoma
Pancreas cancer
Precancer
Rrotein expression
Receptor affinity
Receptor binding
Adenocarcinoma
Animals
Carcinogens
Cholecystokinin
Cricetinae
Mesocricetus
Nitrosamines
Pancreas
Pancreatic Neoplasms
Precancerous Conditions
Receptors, Cholecystokinin
Receptors, G-Protein-Coupled
Receptors, Gastrointestinal Hormone
Receptors, Somatostatin
Receptors, Vasoactive Intestinal Peptide
Secretin
Somatostatin
Vasoactive Intestinal Peptide
To reference this document use:
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DOI
https://doi.org/10.1093/carcin/17.10.2171
TNO identifier
68913
Source
Carcinogenesis, 17 (10), 2171-2175
Document type
article