Title
Fourier transform infrared spectroscopy with a sample deposition interface as a quantitative detector in size-exclusion chromatography
Author
Kok, S.J.
Arentsen, N.C.
Cools, P.J.C.H.
Hankemeier, Th.
Schoenmakers, P.J.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
2002
Abstract
The use of a state-of-the-art commercial solvent-elimination interface for liquid chromatography-infrared spectroscopy is discussed from the perspective of quantitative analysis. The effect of eluent flow-rate is investigated with respect to the homogeneity of the deposit and the trace width along the deposition trace. Low flow-rates (50 μl/min or less) turn out to be favorable for obtaining a good trace and a high sensitivity. The trace width decreased from 2.20 to 1.13 mm when the flow-rate was reduced from 500 to 25 μl/min. Preservation of chromatographic detail during deposition was evaluated at different substrate moving speeds. The additional (extra-column) band broadening that is inherent to nebulizer-deposition interfaces, causes a loss in resolution in size-exclusion chromatography (SEC)-Fourier transform infrared (FTIR) spectroscopy in comparison with SEC-UV. The repeatability of the deposition is evaluated by SEC-FTIR analyses of polystyrene standards (peak molar-masses: 1250-2 950 000 g/mol) and the RSD is found to range from 3.2 to 5.8% in response and from 0.21 to 0.47% in retention time. © 2002 Elsevier Science B.V. All rights reserved. Chemicals/CAS: Indicators and Reagents; Polystyrenes; Solvents
Subject
Nutrition
Chromatography, Gel
Indicators and Reagents
Polystyrenes
Reference Standards
Reproducibility of Results
Solvents
Spectrophotometry, Ultraviolet
Spectroscopy, Fourier Transform Infrared
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DOI
https://doi.org/10.1016/s0021-9673(01)01424-8
TNO identifier
72665
ISSN
0021-9673
Source
Journal of Chromatography A,, 948 (1-2), 257-265
Document type
article