Title
Effects of consumption of Brussels sprouts on plasma and urinary glutathione S-transferase class-α and -π in humans
Author
Nijhoff, W.A.
Mulder, T.P.J.
Verhagen, H.
van Poppel, G.
Peters, W.H.M.
Publication year
1995
Abstract
The effects of consumption of glucosinolate-containing Brussels sprouts on plasma and urinary glutathione S-transferase (GST) class-α and -π were investigated. Five male and five female non-smoking volunteers were randomly assigned to two groups in a crossover design. Five persons started on a glucosinolate-free diet (control period), while the other five consumed 300 g of cooked Brussels sprouts per day, at the expense of 300 g of glucosinolate-free vegetables (sprouts period). Dietary regimes were reversed after 1 week, GST levels were measured by enzyme-linked immunoabsorbent assay. At the end of the sprouts period, a significant increase (1.5-fold) in plasma class-α GST levels was observed in males but not in females (control versus sprouts, paired t-test; P-values 0.031 and 0.317 respectively), while plasma GST class-π levels as well as secretion of urinary GST class-α and -π levels remained unchanged. We conclude that (i) increased plasma GST class-α levels in males originate probably solely from the liver and not from stomach, intestine or kidney; (ii) males are more susceptible for induction of hepatic GSTs than females; and (iii) urinary GST concentration seems less useful as a biomarker for hepatic GST induction.
Subject
glucosinolate
glutathione transferase
adult
article
Brassica
controlled study
diet
enzyme blood level
enzyme linked immunosorbent assay
female
human
male
priority journal
sex difference
urine level
vegetable
Adult
Biological Markers
Brassica
Cross-Over Studies
Diet
Enzyme Induction
Female
Glucosinolates
Glutathione Transferase
Human
Isoenzymes
Male
Sex Factors
Vegetables
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TNO identifier
232966
ISSN
0143-3334
Source
Carcinogenesis, 16 (4), 955-957
Document type
article