Title
A dietary mixture containing fish oil, resveratrol, lycopene, catechins, and vitamins E and C reduces atherosclerosis in transgenic mice
Author
Verschuren, L.
Wielinga, P.Y.
van Duyvenvoorde, W.
Tijani, S.
Toet, K.
van Ommen, B.
Kooistra, T.
Kleemann, R.
Publication year
2011
Abstract
Chronic inflammation and proatherogenic lipids are important risk factors of cardiovascular disease (CVD). Specific dietary constituents such as polyphenols and fish oils may improve cardiovascular risk factors and may have a beneficial effect on disease outcomes. We hypothesized that the intake of an antiinflammatory dietary mixture (AIDM) containing resveratrol, lycopene, catechin, vitamins E and C, and fish oil would reduce inflammatory risk factors, proatherogenic lipids, and endpoint atherosclerosis. AIDM was evaluated in an inflammation model, male human C-reactive protein (CRP) transgenic mice, and an atherosclerosis model, female ApoE*3Leiden transgenic mice. Two groups of male human-CRP transgenic mice were fed AIDM [0.567% (wt:wt) powder and 0.933% (wt:wt oil)] or placebo for 6 wk. The effects of AIDM on basal and IL-1ß-stimulated CRP expression were investigated. AIDM reduced cytokine-induced human CRP and fibrinogen expression in human-CRP transgenic mice. In the atherosclerosis study, 2 groups of female ApoE*3Leiden transgenic mice were fed an atherogenic diet supplemented with AIDM [0.567% (wt:wt) powder and 0.933% (wt:wt oil)] or placebo for 16 wk. AIDM strongly reduced plasma cholesterol, TG, and serum amyloid A concentrations compared with placebo. Importantly, long-term treatment of ApoE*3Leiden mice with AIDM markedly reduced the development of atherosclerosis by 96% compared with placebo. The effect on atherosclerosis was paralleled by a reduced expression of the vascular inflammation markers and adhesion molecules inter-cellular adhesion molecule-1 and E-selectin. Dietary supplementation of AIDM improves lipid and inflammatory risk factors of CVD and strongly reduces atherosclerotic lesion development in female transgenic mice. © 2011 American Society for Nutrition.
Subject
Life
MSB - Microbiology and Systems Biology MHR - Metabolic Health Research
EELS - Earth, Environmental and Life Sciences
Nutrition
alpha tocopherol
apolipoprotein E3
ascorbic acid
biological marker
C reactive protein
catechin
cell adhesion molecule
cholesterol
cytokine
docosahexaenoic acid
endothelial leukocyte adhesion molecule 1
epigallocatechin gallate
fibrinogen
fish oil
green tea extract
icosapentaenoic acid
intercellular adhesion molecule 1
interleukin 1beta
lipid
lycopene
omega 3 700
placebo
polyunsaturated fatty acid
resveratrol
serum amyloid A
triacylglycerol
unclassified drug
animal experiment
animal model
antiinflammatory dietary mixture
article
atherogenic diet
atherosclerosis
cholesterol blood level
controlled study
diet supplementation
dietary intake
drug efficacy
drug megadose
feeding
female
inflammation
long term care
male
mouse
nonhuman
powder
protein blood level
protein expression
risk factor
risk reduction
transgenic animal
treatment duration
treatment outcome
triacylglycerol blood level
vasculitis
omega 3 700
Solgar Vitamin and Herb
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DOI
https://doi.org/10.3945/jn.110.133751
TNO identifier
429731
ISSN
0022-3166
Source
Journal of Nutrition, 141 (5), 863-869
Document type
article