Print Email Facebook Twitter Analysis of recombinant mycobacteria as T helper type 1 vaccines in an allergy challenge model Title Analysis of recombinant mycobacteria as T helper type 1 vaccines in an allergy challenge model Author TNO Preventie en Gezondheid Janssen, R. Kruisselbrink, A. Hoogteijling, L. Lamb, J.R. Young, D.B. Thole, J.E.R. Publication year 2001 Abstract The potential for development of mycobacteria as T helper type 1 (Th1) vaccines capable of induction of Th1 responses to recombinant antigens was explored in a model system based on an immunodominant peptide from house dust mite. Different recombinant mycobacterial preparations were compared for their ability to induce a Th1 response to the peptide. It was found that mycobacterial viability was not a prerequisite for Th1 immunogenicity. A dominant interferon-γ (IFN-γ) response to peptide was observed in splenocytes from C57BL/6J mice immunized with live or heat-killed preparations of recombinant Mycobacterium vaccae or with live attenuated bacillus Calmette-Guèrin (BCG) vaccine expressing the antigen. Interleukin-5 (IL-5), a marker of a Th2 response, was detected only in mice receiving live M. vaccae. A similar pattern was observed in BALB/b mice, although the magnitude of the IFN-γ response was much lower. Control and immunized mice were subsequently exposed to allergen using a Th2-inducing challenge protocol. A significant shift from a Th2 to a Th1 response was observed in immunized mice, as judged by cytokine expression by splenocytes and by subclass of circulating antibody. The effect was seen in three inbred mouse strains differing in their innate bias towards Th1 or Th2 responses. It was dependent on the presence of specific antigen in the mycobacterial preparation and, under the immunization conditions tested, was more pronounced with dead M. vaccae than with live BCG as carrier vaccine. The results demonstrate the potency of killed mycobacteria as Th1 adjuvants and suggest a potential application for recombinant mycobacteria in antigen-specific immune modulation. Chemicals/CAS: Adjuvants, Immunologic; Allergens; Antigens, Dermatophagoides; Bacterial Vaccines; Glycoproteins; Immunoglobulin G; Interferon Type II, 82115-62-6; Vaccines, Synthetic Subject antibodyBCG vaccineepitopegamma interferoninterleukin 5live vaccinerecombinant antigenrecombinant vaccineanimal cellanimal experimentcontrolled studyimmune responseimmunizationimmunogenicityimmunomodulationmousenonhumanspleen cellTh1 cellTh2 cellAdjuvants, ImmunologicAllergensAnimalsAntigens, DermatophagoidesBacterial VaccinesFemaleGlycoproteinsImmunoglobulin GInterferon Type IIMiceMice, Inbred BALB CMice, Inbred C57BLMitesMycobacteriumMycobacterium bovisSpecies SpecificitySpleenTh1 CellsTh2 CellsVaccines, Synthetic To reference this document use: http://resolver.tudelft.nl/uuid:b4502955-24b4-4ac4-aba9-039227bf3057 DOI https://doi.org/10.1046/j.1365-2567.2001.01207.x TNO identifier 280365 ISSN 0019-2805 Source Immunology, 102 (102), 441-449 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.