Print Email Facebook Twitter Hepatic glucose production is more sensitive to insulin-mediated inhibition than hepatic VLDL-triglyceride production Title Hepatic glucose production is more sensitive to insulin-mediated inhibition than hepatic VLDL-triglyceride production Author den Boer, M.A.M. Voshol, P.J. Kuipers, F. Romijn, J.A. Havekes, L.M. TNO Kwaliteit van Leven Publication year 2006 Abstract Insulin is an important inhibitor of both hepatic glucose output and hepatic VLDL-triglyceride (VLDL-TG) production. We investigated whether both processes are equally sensitive to insulin-mediated inhibition. To test this, we used euglycemic clamp studies with four increasing plasma concentrations of insulin in wild-type C57Bl/6 mice. By extrapolation, we estimated that half-maximal inhibition of hepatic glucose output and hepatic VLDL-TG production by insulin were obtained at plasma insulin levels of ∼3.6 and ∼6.8 ng/ml, respectively. In the same experiments, we measured that half-maximal decrease of plasma free fatty acid levels and half-maximal stimulation of peripheral glucose uptake were reached at plasma insulin levels of ∼3.0 and ∼6.0 ng/ml, respectively. We conclude that, compared with insulin sensitivity of hepatic glucose output, peripheral glucose uptake and hepatic VLDL-TG production are less sensitive to insulin. Copyright © 2006 the American Physiological Society. Chemicals / CAS: insulin, 9004-10-8; Blood Glucose; Fatty Acids, Nonesterified; Glucose, 50-99-7; Hypoglycemic Agents; Insulin, 11061-68-0; Lipoproteins, VLDL; Triglycerides Subject BiologyBiomedical ResearchEuglycemic hyperinsulinemic clampFatty acidVery-low-density lipoproteinInsulinAdipose tissueAnimal experimentAanimal modelControlled studyDrug effectDrug inhibitionFatty acid blood levelGluconeogenesisGlucose infusionGlucose transportHyperinsulinemiaInsulin blood levelInsulin resistanceInsulin sensitivityLipolysisLiver functionMouseNonhumanAnimalsBlood GlucoseDose-Response Relationship, DrugFatty Acids, NonesterifiedGlucoseGlucose Clamp TechniqueHypoglycemic AgentsInsulinKineticsLipoproteins, VLDLLiverMaleMiceMice, Inbred C57BLTriglycerides To reference this document use: http://resolver.tudelft.nl/uuid:b2ff3d86-d5f9-4c0c-9581-e4ea3f648051 DOI https://doi.org/10.1152/ajpendo.00188.2006 TNO identifier 239769 ISSN 0193-1849 Source American Journal of Physiology - Endocrinology and Metabolism, 291 (6) Document type article Files To receive the publication files, please send an e-mail request to TNO Library.