Title
Role of bacterial virulence proteins in Agrobacterium-mediated transformation of Aspergillus awamori
Author
Michielse, C.B.
Ram, A.F.J.
Hooykaas, P.J.J.
van den Hondel, C.A.M.J.J.
TNO Voeding
Publication year
2004
Abstract
The Agrobacterium-mediated transformation of Aspergillus awamori was optimized using defined co-cultivation conditions, which resulted in a reproducible and efficient transformation system. Optimal co-cultivation conditions were used to study the role of Agrobacterium tumefaciens virulence proteins in T-DNA transfer. This study revealed that inactivation of either of the regulatory proteins (VirA, VirG), any of the transport pore proteins (VirB), proteins involved in generation of the T-strand (VirD, VirC) or T-strand protection and targeting (VirE2) abolishes or severely reduces the formation of transformants. The results indicate that the Agrobacterium-mediated transformation of A. awamori requires an intact T-DNA machinery for efficient transformation; however, the plant host range factors, like VirE3, VirH, and VirF, are not important. Index descriptors: Agrobacterium tumefaciens; T-DNA; Virulence proteins; DNA-transformation; Aspergillus awamori; Filamentous fungi © 2004 Elsevier Inc. All rights reserved.
Subject
Biology
Biotechnology
bacterial protein
protein VirA
protein virC
protein virD
protein virG
regulator protein
unclassified drug
article
Aspergillus awamori
bacterial virulence
biotransformation
controlled study
cultivar
DNA strand
DNA transfer
host pathogen interaction
nonhuman
plant
priority journal
protein targeting
reaction optimization
reduction
reproducibility
Rhizobium
system analysis
Aspergillus
Bacterial Proteins
Conjugation, Genetic
DNA, Bacterial
DNA-Binding Proteins
Gene Deletion
Genes, Bacterial
Ion Channels
Mutagenesis, Insertional
Plant Tumor-Inducing Plasmids
Rhizobium radiobacter
Temperature
Time Factors
Transformation, Genetic
Virulence Factors
Agrobacterium
Agrobacterium tumefaciens
Aspergillus
Aspergillus awamori
Bacteria (microorganisms)
Viruses (infective agents)
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DOI
https://doi.org/10.1016/j.fgb.2004.01.004
TNO identifier
237732
ISSN
1087-1845
Source
Fungal Genetics and Biology, 41 (5), 571-578
Document type
article