Title
Banned antibacterial growth promoters in animal feed: Collaborative trial on the liquid chromatography-tandem mass spectrometry method developed in the feedstuffs-radius project
Author
Poucke, C.V.
Dumoulin, F.
Yakkundi, S.
Situ, C.
Elliott, C.T.
Grutters, E.M.
Verheijen, R.
Schilt, R.
Eriksson, S.
Peteghem, C.V.
TNO Kwaliteit van Leven
Publication year
2006
Abstract
A chemical confirmation method for the identification and quantification of five banned antibacterial growth promoters (AGPs) in animal feed was developed and in-house validated as part of the European Feedstuffs-RADIUS project [1]. To complete the validation process a collaborative trial was performed by eleven laboratories within the European Union (EU) using the developed method. Laboratories were supplied with six animal feed samples. None of the participants reported any false non-compliant results on these samples. Neither where there any false compliant results for four out of five compounds. These data proved the procedure to be robust and an excellent means of controlling misuse of the banned antibacterial growth promoters. During a small feed survey, a selection (n = 614) of animal feed samples were screened with a multi-antibiotic ELISA method [2] that was developed in the same EU project and non-compliant samples were submitted to liquid chromatography-tandem mass spectrometry (LC-MS/MS) confirmation. The results from this confirmatory analysis indicated that the LC-MS/MS method will be a valuable tool in helping to determine the origin of the detected antibacterial compounds and that animals are still receiving tylosin in concentrations that are in the same range as the now banned AGP concentrations. © 2005 Elsevier B.V. All rights reserved.
Subject
Biology
Analytical research
Antibacterial growth promoters
Collaborative trial
Cross-contamination
Feedstuffs survey
LC-MS/MS
Medicated feed
Antibiotics
Data reduction
Liquid chromatography
Mass spectrometry
Antibacterial growth promoters
Collaborative trial
Cross-contamination
Feedstuffs surveys
LC-MS/MS
Medicated stuffs
Growth kinetics
bacitracin
growth promotor
olaquindox
spiramycin
tylosin
virginiamycin
animal food
chemical analysis
conference paper
enzyme linked immunosorbent assay
experimental animal
laboratory
liquid chromatography
priority journal
tandem mass spectrometry
Animalia
To reference this document use:
http://resolver.tudelft.nl/uuid:b0ca6cea-7711-4fd1-8d16-0916677e41c7
DOI
https://doi.org/10.1016/j.aca.2005.09.071
TNO identifier
239098
ISSN
0003-2670
Source
Analytica Chimica Acta, 557 (1-2), 204-210
Document type
article