Print Email Facebook Twitter Effect of low dose atorvastatin versus diet-induced cholesterol lowering on atherosclerotic lesion progression and inflammation in apolipoprotein E*3-Leiden transgenic mice Title Effect of low dose atorvastatin versus diet-induced cholesterol lowering on atherosclerotic lesion progression and inflammation in apolipoprotein E*3-Leiden transgenic mice Author Verschuren, L. Kleemann, R. Offerman, E.H. Szalai, A.J. Emeis, S.J. Princen, H.M.G. Kooistra, T. TNO Kwaliteit van Leven Publication year 2005 Abstract Objective - To evaluate whether low-dose atorvastatin suppresses atherosclerotic lesion progression and inflammation in apolipoprotein E*3 (apoE*3)-Leiden mice beyond its cholesterol-lowering effect. Methods and Results - ApoE*3-Leiden mice were fed a high-cholesterol (HC) diet until mild atherosclerotic lesions had formed. Subsequently, HC diet feeding was continued or mice received HC supplemented with 0.002% (w/w) atorvastatin (HC+A), resulting in 19% plasma cholesterol lowering, or mice received a low-cholesterol (LC) diet to establish a plasma cholesterol level similar to that achieved in the HC+A group. HC+A and LC diet reduced, significantly and to the same extent, lesion progression and complication in the aortic root, as assessed by measuring total atherosclerotic lesion area, lesion severity, and macrophage and smooth muscle cell area. In the aortic arch, HC+A but not LC blocked lesion progression. HC+A and LC reduced vascular inflammation (ie, expression of macrophage migration inhibitory factor, plasminogen activator inhibitor-1, matrix metalloproteinase-9), but HC+A additionally suppressed vascular cell adhesion molecule-1 expression and, in parallel, monocyte adhesion. In contrast, low-dose atorvastatin showed no antiinflammatory action toward hepatic inflammation markers (serum amyloid A, C-reactive protein [CRP]) in apoE*3-Leiden mice and human CRP transgenic mice. Conclusion - Low-dose atorvastatin cholesterol-dependently reduces lesion progression in the aortic root but shows antiinflammatory vascular activity and tends to retard atherogenesis in the aortic arch beyond its cholesterol-lowering effect. Chemicals / CAS: amyloid A protein, 59165-71-8; atorvastatin, 134523-00-5, 134523-03-8; C reactive protein, 9007-41-4; cholesterol, 57-88-5; gelatinase B, 146480-36-6; plasminogen activator inhibitor 1, 140208-23-7; Anticholesteremic Agents; apolipoprotein E3 (Leidein); Apolipoprotein E3; Apolipoproteins E; atorvastatin, 110862-48-1; Biological Markers; C-Reactive Protein, 9007-41-4; Cholesterol, 57-88-5; Heptanoic Acids; Pyrroles; Serum Amyloid A Protein Subject BiologyBiomedical ResearchGrowth factorsLipidsLipoprotein metabolismPathophysiologyC reactive proteinCholesterolGelatinase BMacrophage migration inhibition factorPlasminogen activator inhibitor 1Vascular cell adhesion molecule 1Animal cellAnimal experimentanimal modelAntiinflammatory activityAorta archCholesterol blood levelCholesterol dietControlled studyDietary intakeDisease activityDisease courseDisease severityInflammationLipoprotein metabolismLow drug doseMacrophageMonocyteMouseNonhumanPathophysiologyProtein expressionSmooth muscle fiberTransgenic mouseTreatment outcomeAmyloid A proteinApolipoprotein E3AtorvastatinAnimalsAnticholesteremic AgentsAortaApolipoprotein E3Apolipoproteins EArteriosclerosisBiological MarkersC-Reactive ProteinCholesterolDietDiet, AtherogenicDrug Administration ScheduleFemaleHeptanoic AcidsInflammationMaleMiceMice, TransgenicPyrrolesSerum Amyloid A Protein To reference this document use: http://resolver.tudelft.nl/uuid:b0c8a00b-b259-48df-8c3c-128e744bd7cd DOI https://doi.org/10.1161/01.atv.0000148866.29829.19 TNO identifier 238267 ISSN 1079-5642 Source Arteriosclerosis, Thrombosis, and Vascular Biology, 25 (1), 161-167 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.