Arachidonic acid/docosahexaenoic acid-supplemented diet in early life reduces body weight gain, plasma lipids, and adiposity in later life in ApoE*3 Leiden mice

Arachidonic acid/docosahexaenoic acid-supplemented diet in early life reduces body weight gain, plasma lipids, and adiposity in later life in ApoE*3 Leiden mice

Wielinga, P.Y.,
Harthoorn, L.F.,
Verschuren, L.,
Schoemaker, M.H.,
Jouni, Z.E.,
van, Tol, E.A.F.
Kleemann, R.,
Kooistra, T.

2012

Scope: This study addresses whether early life arachidonic acid (ARA)/docosahexaenoic acid (DHA) supplementation or eicosapentaenoic acid (EPA)/DHA (Omacor) supplementation affects body weight gain, lipid metabolism, and adipose tissue quantity and quality in later life in ApoE*3Leiden-transgenic mice, a humanized model for hyperlipidemia and mild obesity. Methods and results: Four-week-old male ApoE*3Leiden mice were fed chow diet with or without a mixture of ARA (0.129 wt%) and DHA (0.088 wt%) or Omacor (0.30 wt% EPA, 0.25 wt% DHA). At age 12 weeks, mice were fed high-fat/high-carbohydrate (HFHC) diet without above supplements until age 20 weeks. Control mice received chow diet throughout the study. Mice receiving ARA/DHA gained less body weight compared to control and this effect was sustained when fed HFHC. Omacor had no significant effect on body weight gain. Plasma cholesterol and triglycerides were significantly lowered by both supplementations. At 20 weeks, epididymal fat mass was less in ARA/DHA-supplemented mice, while Omacor had no significant effect on fat mass. Both ARA/DHA and Omacor reduced inguinal adipocyte cell size; only ARA/DHA significantly reduced epididymal macrophage infiltration. Conclusion: This study shows that early life ARA/DHA, but not Omacor supplementation improves body weight gain later in life. ARA/DHA and to a lesser extentOmacor both improved adipose tissue quality. Chemicals / CAS arachidonic acid, 506-32-1, 6610-25-9, 7771-44-0; cholesterol, 57-88-5; docosahexaenoic acid, 25167-62-8, 32839-18-2; Anti-Obesity Agents; Apolipoprotein E3; Arachidonic Acid, 506-32-1; Cholesterol, 57-88-5; Docosahexaenoic Acids, 25167-62-8; Hypolipidemic Agents; Triglycerides; apolipoprotein E3 (Leidein)

Adipose Tissue, White
Adiposity
Animals
Anti-Obesity Agents
Apolipoprotein E3
Arachidonic Acid
Cell Size
Cholesterol
Dietary Supplements
Docosahexaenoic Acids
Hyperlipidemias
Hypolipidemic Agents
Macrophages
Male
Mice
Mice, Transgenic
Obesity
Specific Pathogen-Free Organisms
Triglycerides
Weight Gain
Mus
Mus musculus
Biomedical Innovation
Healthy Living
Life
MHR - Metabolic Health Research
EELS - Earth, Environmental and Life Sciences

http://resolver.tudelft.nl/uuid:ac55007d-8de4-40a1-8866-7109d1eaba5f

https://doi.org/10.1002/mnfr.201100762

461910

Molecular Nutrition & Food Research, 56 (56), 1081-1089

article