Print Email Facebook Twitter Determination of N7-methylguanine in DNA of white blood cells from cancer patients treated with dacarbazine Title Determination of N7-methylguanine in DNA of white blood cells from cancer patients treated with dacarbazine Author van Delft, J.H.M. van den Ende, A.M.C. Keizer, H.J. Ouwerkerk, J. Baan, R.A. Centraal Instituut voor Voedingsonderzoek TNO Medisch Biologisch Laboratorium TNO Publication year 1992 Abstract An ELISA method was used to determine N7-methylguanine (N7-MeGua) adducts in DNA of white blood cells from cancer patients treated with the methylating antitumor drug dacarbazine by i.v. administration. From four patients who received dacarbazine only, at dosages of 250, 400 or 800 mg/m2, the blood samples were collected before and several days after administration, and from one patient also during the first few hours. N7-MeGua levels increased rapidly during the first hour after treatment, hardly changed during the following 7 h and decreased during the next days (half-life of ~ 72 h). The adduct levels appeared to be dose-dependent. Blood cells were also analyzed from three patients who received dacarbazine (225 mg/m2) in combination with other chemotherapeutic drugs during three successive days. One of these patients showed a larger initial level of N7-MeGua and a cumulative increase with the dacarbazine dose compared to the other two patients. For several patients adduct levels were monitored during two chemotherapy cycles, for which quite similar data were obtained. Chemicals/CAS: 7 methylguanine, 578-76-7; carmustine, 154-93-8; cisplatin, 15663-27-1, 26035-31-4, 96081-74-2; dacarbazine, 4342-03-4; 7-methylguanine, 578-76-7; Antineoplastic Combined Chemotherapy Protocols; Dacarbazine, 4342-03-4; DNA, 9007-49-2; Guanine, 73-40-5 Subject 7 MethylguanineCarmustineCisplatinDacarbazineBlood samplingCancer patientCell levelClinical articleDNA determinationDose responseHuman cellIntravenous drug administrationPatient monitoringAdultAntineoplastic Combined Chemotherapy ProtocolsDacarbazineDNAEnzyme-Linked Immunosorbent AssayFemaleGuanineHumanLeukocytesMaleMelanomaMiddle AgeSarcomaSupport, Non-U.S. To reference this document use: http://resolver.tudelft.nl/uuid:aab6259f-5e1d-4a58-b64c-ccaf99ffd650 TNO identifier 67452 Source Carcinogenesis, 13 (7), 1257-1959 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.