Title
Optimization of the solvent-tolerant Pseudomonas putida S12 as host for the production of p-coumarate from glucose
Author
Nijkamp, K.
Westerhof, R.G.M.
Ballerstedt, H.
de Bont, J.A.M.
Wery, J.
TNO Kwaliteit van Leven
Publication year
2007
Abstract
A Pseudomonas putida S12 strain was constructed that is able to convert glucose to p-coumarate via the central metabolite l-tyrosine. Efficient production was hampered by product degradation, limited cellular L-tyrosine availability, and formation of the by-product cinnamate via L-phenylalanine. The production host was optimized by inactivation of fcs, the gene encoding the first enzyme in the p-coumarate degradation pathway in P. putida, followed by construction of a phenylalanine-auxotrophic mutant. These steps resulted in a P. putida S12 strain that showed dramatically enhanced production characteristics with controlled l-phenylalanine feeding. During fed-batch cultivation, 10 mM (1.7 g l-1) of p-coumarate was produced from glucose with a yield of 3.8 Cmol% and a molar ratio of p-coumarate to cinnamate of 85:1. © 2006 Springer-Verlag.
Subject
Biology
Biotechnology
Degradation
Feeding
Genes
Genetic engineering
Glucose
Mutagenesis
Optimization
Production control
Solvents
Cellular L-tyrosine
Molar ratio
p-coumarate
Solvent-tolerant Pseudomonas putida
Bacteria
cinnamic acid
glucose
para coumaric acid
phenylalanine
tyrosine
amino acid
bacterium
biodegradation
degradation
enzyme
glucose
metabolite
optimization
organic acid
article
controlled study
feeding
host
mutant
nonhuman
Pseudomonas putida
Cinnamates
Coenzyme A Ligases
Coumaric Acids
Fermentation
Gene Deletion
Glucose
Phenylalanine
Pseudomonas putida
Tyrosine
Pseudomonas putida
To reference this document use:
http://resolver.tudelft.nl/uuid:aa330609-aa1a-4fb7-90f0-ed510ddaa7ea
DOI
https://doi.org/10.1007/s00253-006-0703-0
TNO identifier
239860
ISSN
0175-7598
Source
Applied Microbiology and Biotechnology, 74 (3), 617-624
Document type
article