Title
Effects of cyclosporin A and cyclophosphamide on Peyer's patches in rat, exposed in utero and neonatally or during adult age
Author
Kuper, C.F.
van Zijverden, M.
Klaassen, C.
Tegelenbosch-Schouten, M.
Wolterbeek, A.P.M.
TNO Kwaliteit van Leven
Publication year
2007
Abstract
The effects of cyclosporin A (CY) and cyclophosphamide (CPS) on Peyer's patches (PP) were studied in Wistar rats, exposed in utero and neonatally or during adult age. In one study, pregnant dams received 5 or 15 mg/kg bw/day CY from gestation day 6 to day 21 of lactation. In two other studies, animals were exposed at young adult age: female rats received orally 5 or 20 mg/kg/day CY or 5 or 10 mg/kg bw CPS for 4 weeks; males received orally 5 mg/kg bw CPS for 4 weeks, or a single iv injection of 50 mg/kg bw CPS. Upon in utero and neonatal exposure, the numbers of grossly observed PP were increased in male pups from the high-dose CY dams at 70 days of age. Exposure to high-dose CY at adult age only tended to decrease the numbers of PP; germinal center development was reduced in the PP from the middle segment of the small intestines, as examined microscopically. Exposure to both doses CPS at adult age reduced the numbers of PP and reduced germinal centre development and the number of lymphocytes in all compartments of PP. It was concluded that the effects of CPS and CY could be established by counting the number of grossly visible PP and by microscopic observation of PP, provided that regional differences of PP were taken into account. Moreover, the type of effects of an immunotoxic agent may vary with age of exposure. Copyright © by the Society of Toxicologic Pathology.
Subject
Biology
Toxicology and Applied Pharmacology
Adult exposure
Cyclophosphamide
Cyclosporin
Fetal exposure
Peyer's patches
Rat
cyclophosphamide
cyclosporin A
animal experiment
animal tissue
article
controlled study
drug effect
female
fetotoxicity
germinal center
gestation period
immunotoxicity
lactation
lymphocyte
male
microscopy
newborn period
nonhuman
Peyer patch
pregnancy
priority journal
rat
small intestine
Wistar rat
Administration, Oral
Aging
Animals
Cell Count
Cyclophosphamide
Cyclosporine
Female
Germinal Center
Immunosuppressive Agents
Injections, Intravenous
Male
Peyer's Patches
Pregnancy
Prenatal Exposure Delayed Effects
Rats
Rats, Wistar
Animalia
Rattus
Rattus norvegicus
To reference this document use:
http://resolver.tudelft.nl/uuid:aa1cc766-4dcb-49a0-bd46-5602655448c6
DOI
https://doi.org/10.1080/01926230601156245
TNO identifier
239896
ISSN
0192-6233
Source
Toxicologic Pathology, 35 (2), 226-232
Document type
article