Print Email Facebook Twitter Mannosylated self-peptide inhibits the development of experimental autoimmune encephalomyelitis via expansion of nonencephalitogenic T cells Title Mannosylated self-peptide inhibits the development of experimental autoimmune encephalomyelitis via expansion of nonencephalitogenic T cells Author Kel, J.M. Slütter, B. Drijfhout, J.W. Koning, F. Nagelkerken, L. TNO Kwaliteit van Leven Publication year 2008 Abstract Tolerance to experimental autoimmune encephalomyelitis (EAE) in SJL mice can be induced by immunization with a mannosylated form of the proteolipid protein (M-PLP139-151), despite the presence of CFA. The state of tolerance is characterized by poor delayed-type hypersensitivity responses and the absence of clinical EAE symptoms. In vivo monitoring of CFSE-labeled PLP139-151-specific TCR-transgenic (5B6) T cells revealed that immunization with M-PLP139-151 increases the clonal expansion of 5B6 T cells that do not develop full effector functions. Moreover, nonfunctional T cells obtained from M-PLP139-151-immunized mice showed poor blastogenesis and were unable to transfer EAE to naïve recipients. Nevertheless, the in vitro production of cytokines and chemokines associated with EAE was unaffected. Importantly, tolerance induced by M-PLP 139-151 was abrogated by the administration of pertussis toxin, resulting in EAE development. Our results suggest that M-PLP139-151 inhibits EAE development by affecting the differentiation of T cells into encephalitogenic effector cells. © Society for Leukocyte Biology. Subject BiologyBiomedical ResearchC-type lectinsDelayed-type hypersensitivity (DTH)Tolerancechemokinecytokinepertussis toxinprotein m plp139 151protein plp139 151proteolipid proteinadoptive transferallergic encephalomyelitisanimal cellanimal experimentantigen specificityarticleautoimmunityCD4+ T lymphocyteclonogenesiscontrolled studycytokine productiondelayed hypersensitivitydrug inhibitiondrug toleranceeffector cellfemaleimmunizationimmunocompetent celllymphocyte transformationmousenonhumanpriority journalT lymphocytetransgenicsAnimalsCell CountCell ProliferationClone CellsEncephalomyelitis, Autoimmune, ExperimentalEpitopesFemaleImmune ToleranceImmunizationMannoseMicePeptidesPertussis ToxinSpleenT-LymphocytesMus To reference this document use: http://resolver.tudelft.nl/uuid:a96f864c-b81c-45aa-a598-69e330939424 DOI https://doi.org/10.1189/jlb.0507312 TNO identifier 240889 ISSN 0741-5400 Source Journal of Leukocyte Biology, 84 (1), 182-190 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.